Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells
Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new methodologies should be developed for this purpose and then submitted to confirmation of isolation by means of seroneutralization.
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Sociedade Brasileira de Microbiologia
2005
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oai:scielo:S1517-838220050003000012006-02-20Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cellsJerez,José AntonioGregori,FábioBrandão,Paulo EduardoRodriguez,Cesar Alejandro RosalesIto,Fumio HonmaBuzinaro,Maria da GlóriaSakai,Takeo bovine coronavirus (BCoV) isolation HmLu-1 cells Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new methodologies should be developed for this purpose and then submitted to confirmation of isolation by means of seroneutralization.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.36 n.3 20052005-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001en10.1590/S1517-83822005000300001 |
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Jerez,José Antonio Gregori,Fábio Brandão,Paulo Eduardo Rodriguez,Cesar Alejandro Rosales Ito,Fumio Honma Buzinaro,Maria da Glória Sakai,Takeo |
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Jerez,José Antonio Gregori,Fábio Brandão,Paulo Eduardo Rodriguez,Cesar Alejandro Rosales Ito,Fumio Honma Buzinaro,Maria da Glória Sakai,Takeo Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells |
author_facet |
Jerez,José Antonio Gregori,Fábio Brandão,Paulo Eduardo Rodriguez,Cesar Alejandro Rosales Ito,Fumio Honma Buzinaro,Maria da Glória Sakai,Takeo |
author_sort |
Jerez,José Antonio |
title |
Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells |
title_short |
Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells |
title_full |
Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells |
title_fullStr |
Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells |
title_full_unstemmed |
Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells |
title_sort |
isolation of bovine coronavirus (bcov) in monolayers of hmlu-1 cells |
description |
Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new methodologies should be developed for this purpose and then submitted to confirmation of isolation by means of seroneutralization. |
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Sociedade Brasileira de Microbiologia |
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2005 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001 |
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