Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis

Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis

In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole structure, except in the carboxyl-terminal region.

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Main Authors: Gibotti,Adriana, Tanaka,Tânia L., Oliveira,Valéria R., Taddei,Carla R., Martinez,Marina B.
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2004
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012
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spelling oai:scielo:S1517-838220040001000122004-11-16Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysisGibotti,AdrianaTanaka,Tânia L.Oliveira,Valéria R.Taddei,Carla R.Martinez,Marina B. ipa enteroinvasive Escherichia coli polymorphism PCR-RFLP In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole structure, except in the carboxyl-terminal region.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.35 n.1-2 20042004-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012en10.1590/S1517-83822004000100012
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Gibotti,Adriana
Tanaka,Tânia L.
Oliveira,Valéria R.
Taddei,Carla R.
Martinez,Marina B.
spellingShingle Gibotti,Adriana
Tanaka,Tânia L.
Oliveira,Valéria R.
Taddei,Carla R.
Martinez,Marina B.
Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
author_facet Gibotti,Adriana
Tanaka,Tânia L.
Oliveira,Valéria R.
Taddei,Carla R.
Martinez,Marina B.
author_sort Gibotti,Adriana
title Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_short Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_full Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_fullStr Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_full_unstemmed Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_sort molecular characterization of enteroinvasive escherichia coli ipa genes by pcr-rflp analysis
description In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole structure, except in the carboxyl-terminal region.
publisher Sociedade Brasileira de Microbiologia
publishDate 2004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012
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AT tanakatanial molecularcharacterizationofenteroinvasiveescherichiacoliipagenesbypcrrflpanalysis
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AT taddeicarlar molecularcharacterizationofenteroinvasiveescherichiacoliipagenesbypcrrflpanalysis
AT martinezmarinab molecularcharacterizationofenteroinvasiveescherichiacoliipagenesbypcrrflpanalysis
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