A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed.
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Sociedade Brasileira de Microbiologia
2000
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:scielo:S1517-83822000000100008 |
|---|---|
| record_format |
ojs |
| spelling |
oai:scielo:S1517-838220000001000082000-08-25A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing mediumNascimento,Aline E.Shariá,Allana E. do NascimentoLima,Marco Antonio Barbosa deCampos-Takaki,Galba Maria Candida lipolytica carbohydrate cytochemistry fungi ultrastructure Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.31 n.1 20002000-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008en10.1590/S1517-83822000000100008 |
| institution |
SCIELO |
| collection |
OJS |
| country |
Brasil |
| countrycode |
BR |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-scielo-br |
| tag |
revista |
| region |
America del Sur |
| libraryname |
SciELO |
| language |
English |
| format |
Digital |
| author |
Nascimento,Aline E. Shariá,Allana E. do Nascimento Lima,Marco Antonio Barbosa de Campos-Takaki,Galba Maria |
| spellingShingle |
Nascimento,Aline E. Shariá,Allana E. do Nascimento Lima,Marco Antonio Barbosa de Campos-Takaki,Galba Maria A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium |
| author_facet |
Nascimento,Aline E. Shariá,Allana E. do Nascimento Lima,Marco Antonio Barbosa de Campos-Takaki,Galba Maria |
| author_sort |
Nascimento,Aline E. |
| title |
A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium |
| title_short |
A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium |
| title_full |
A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium |
| title_fullStr |
A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium |
| title_full_unstemmed |
A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium |
| title_sort |
cytochemical study of acid carbohydrates on the surface of candida lipolytica grown in tween 80-containing medium |
| description |
Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed. |
| publisher |
Sociedade Brasileira de Microbiologia |
| publishDate |
2000 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008 |
| work_keys_str_mv |
AT nascimentoalinee acytochemicalstudyofacidcarbohydratesonthesurfaceofcandidalipolyticagrownintween80containingmedium AT shariaallanaedonascimento acytochemicalstudyofacidcarbohydratesonthesurfaceofcandidalipolyticagrownintween80containingmedium AT limamarcoantoniobarbosade acytochemicalstudyofacidcarbohydratesonthesurfaceofcandidalipolyticagrownintween80containingmedium AT campostakakigalbamaria acytochemicalstudyofacidcarbohydratesonthesurfaceofcandidalipolyticagrownintween80containingmedium AT nascimentoalinee cytochemicalstudyofacidcarbohydratesonthesurfaceofcandidalipolyticagrownintween80containingmedium AT shariaallanaedonascimento cytochemicalstudyofacidcarbohydratesonthesurfaceofcandidalipolyticagrownintween80containingmedium AT limamarcoantoniobarbosade cytochemicalstudyofacidcarbohydratesonthesurfaceofcandidalipolyticagrownintween80containingmedium AT campostakakigalbamaria cytochemicalstudyofacidcarbohydratesonthesurfaceofcandidalipolyticagrownintween80containingmedium |
| _version_ |
1756424779430100992 |