A modified pH-cycling model to evaluate fluoride effect on enamel demineralization
Since in vitro pH-cycling models are widely used to study dental caries, they should allow evaluations of fluoride effect on early stages of caries development. Therefore, acid etching on enamel surface must be avoided, enabling surface microhardness (SMH) analysis. In the present study, the pH-cycling model originally described by Featherstone et al.9 (1986) was modified to preserve the enamel surface and to produce early carious lesions that could be evaluated using SMH and cross-sectional microhardness (CSMH) measurements. In order to validate this modified model, a dose-response evaluation with fluoride was made. Human enamel blocks with known SMH were submitted to such regimen with the following treatments: distilled deionized water (DDW; control) and solutions containing 70, 140 and 280 ppm F. Data from %SMH change and deltaZ (mineral loss) showed a statistically significant negative correlation between F concentration in treatment solutions and mineral loss. In conclusion, the modified pH-cycling model allowed the evaluation of changes on the outermost enamel layer during caries development, and a dose-response effect of fluoride reducing enamel demineralization was observed.
Main Authors: | , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Pesquisa Odontológica e Faculdade de Odontologia da Universidade de São Paulo
2003
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-74912003000300008 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S1517-74912003000300008 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S1517-749120030003000082003-12-16A modified pH-cycling model to evaluate fluoride effect on enamel demineralizationArgenta,Rosane Maria OrthTabchoury,Cinthia Pereira MachadoCury,Jaime Aparecido Dental enamel Demineralization Fluorine Fluorides Since in vitro pH-cycling models are widely used to study dental caries, they should allow evaluations of fluoride effect on early stages of caries development. Therefore, acid etching on enamel surface must be avoided, enabling surface microhardness (SMH) analysis. In the present study, the pH-cycling model originally described by Featherstone et al.9 (1986) was modified to preserve the enamel surface and to produce early carious lesions that could be evaluated using SMH and cross-sectional microhardness (CSMH) measurements. In order to validate this modified model, a dose-response evaluation with fluoride was made. Human enamel blocks with known SMH were submitted to such regimen with the following treatments: distilled deionized water (DDW; control) and solutions containing 70, 140 and 280 ppm F. Data from %SMH change and deltaZ (mineral loss) showed a statistically significant negative correlation between F concentration in treatment solutions and mineral loss. In conclusion, the modified pH-cycling model allowed the evaluation of changes on the outermost enamel layer during caries development, and a dose-response effect of fluoride reducing enamel demineralization was observed.info:eu-repo/semantics/openAccessSociedade Brasileira de Pesquisa Odontológica e Faculdade de Odontologia da Universidade de São PauloPesquisa Odontológica Brasileira v.17 n.3 20032003-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-74912003000300008en10.1590/S1517-74912003000300008 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Argenta,Rosane Maria Orth Tabchoury,Cinthia Pereira Machado Cury,Jaime Aparecido |
spellingShingle |
Argenta,Rosane Maria Orth Tabchoury,Cinthia Pereira Machado Cury,Jaime Aparecido A modified pH-cycling model to evaluate fluoride effect on enamel demineralization |
author_facet |
Argenta,Rosane Maria Orth Tabchoury,Cinthia Pereira Machado Cury,Jaime Aparecido |
author_sort |
Argenta,Rosane Maria Orth |
title |
A modified pH-cycling model to evaluate fluoride effect on enamel demineralization |
title_short |
A modified pH-cycling model to evaluate fluoride effect on enamel demineralization |
title_full |
A modified pH-cycling model to evaluate fluoride effect on enamel demineralization |
title_fullStr |
A modified pH-cycling model to evaluate fluoride effect on enamel demineralization |
title_full_unstemmed |
A modified pH-cycling model to evaluate fluoride effect on enamel demineralization |
title_sort |
modified ph-cycling model to evaluate fluoride effect on enamel demineralization |
description |
Since in vitro pH-cycling models are widely used to study dental caries, they should allow evaluations of fluoride effect on early stages of caries development. Therefore, acid etching on enamel surface must be avoided, enabling surface microhardness (SMH) analysis. In the present study, the pH-cycling model originally described by Featherstone et al.9 (1986) was modified to preserve the enamel surface and to produce early carious lesions that could be evaluated using SMH and cross-sectional microhardness (CSMH) measurements. In order to validate this modified model, a dose-response evaluation with fluoride was made. Human enamel blocks with known SMH were submitted to such regimen with the following treatments: distilled deionized water (DDW; control) and solutions containing 70, 140 and 280 ppm F. Data from %SMH change and deltaZ (mineral loss) showed a statistically significant negative correlation between F concentration in treatment solutions and mineral loss. In conclusion, the modified pH-cycling model allowed the evaluation of changes on the outermost enamel layer during caries development, and a dose-response effect of fluoride reducing enamel demineralization was observed. |
publisher |
Sociedade Brasileira de Pesquisa Odontológica e Faculdade de Odontologia da Universidade de São Paulo |
publishDate |
2003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-74912003000300008 |
work_keys_str_mv |
AT argentarosanemariaorth amodifiedphcyclingmodeltoevaluatefluorideeffectonenameldemineralization AT tabchourycinthiapereiramachado amodifiedphcyclingmodeltoevaluatefluorideeffectonenameldemineralization AT curyjaimeaparecido amodifiedphcyclingmodeltoevaluatefluorideeffectonenameldemineralization AT argentarosanemariaorth modifiedphcyclingmodeltoevaluatefluorideeffectonenameldemineralization AT tabchourycinthiapereiramachado modifiedphcyclingmodeltoevaluatefluorideeffectonenameldemineralization AT curyjaimeaparecido modifiedphcyclingmodeltoevaluatefluorideeffectonenameldemineralization |
_version_ |
1756424734631788544 |