Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy
Abstract The Paranaguá Estuarine Complex (PEC) is one of Brazil's largest southwest Atlantic estuarine systems, possessing a rich microalgae diversity that remains to be fully explored. Therefore, due to the increasing interest in the microalgae biotechnological potential, this study isolated and identified diatoms found in the PEC. The diatoms were purified and analyzed with light and scanning electron microscopy for morphological identification, while DNA sequences were used for molecular identification. Although a diatoms rich diversity was obtained, only a few were viable after the cultivation period. The two best-selected strains were identified as belonging to two genera, Nitzschia and Navicula. The rbcL region was found to be the most informative for species identification. Morphological and molecular analyses allowed for the identification of species Nitzschia inconspicua and Navicula pseudoantonii, which was understood as the first report of N. pseudoantonii in the American continent.
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Instituto de Tecnologia do Paraná - Tecpar
2023
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oai:scielo:S1516-891320230001004022022-09-13Paranaguá Estuarine Complex Diatoms: Morphology and Molecular TaxonomyRampinelli,Viviane de Fátima Moura AguillarConceição,DanieleSantos,BeatrizMarra,RaquelPassos,Matheus FelipePetersen,RodolfoVargas,José Viriato CoelhoGalli-Terasawa,Lygia VitóriaSavi,DaianiLudwig,Thelma Alvim VeigaFerreira,Paula CarolinaKava,Vanessa bioprospection DNA-barcode rbcL Navicula pseudoantonii Nitzschia inconspicua Abstract The Paranaguá Estuarine Complex (PEC) is one of Brazil's largest southwest Atlantic estuarine systems, possessing a rich microalgae diversity that remains to be fully explored. Therefore, due to the increasing interest in the microalgae biotechnological potential, this study isolated and identified diatoms found in the PEC. The diatoms were purified and analyzed with light and scanning electron microscopy for morphological identification, while DNA sequences were used for molecular identification. Although a diatoms rich diversity was obtained, only a few were viable after the cultivation period. The two best-selected strains were identified as belonging to two genera, Nitzschia and Navicula. The rbcL region was found to be the most informative for species identification. Morphological and molecular analyses allowed for the identification of species Nitzschia inconspicua and Navicula pseudoantonii, which was understood as the first report of N. pseudoantonii in the American continent.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.66 20232023-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100402en10.1590/1678-4324-2023210523 |
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Rampinelli,Viviane de Fátima Moura Aguillar Conceição,Daniele Santos,Beatriz Marra,Raquel Passos,Matheus Felipe Petersen,Rodolfo Vargas,José Viriato Coelho Galli-Terasawa,Lygia Vitória Savi,Daiani Ludwig,Thelma Alvim Veiga Ferreira,Paula Carolina Kava,Vanessa |
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Rampinelli,Viviane de Fátima Moura Aguillar Conceição,Daniele Santos,Beatriz Marra,Raquel Passos,Matheus Felipe Petersen,Rodolfo Vargas,José Viriato Coelho Galli-Terasawa,Lygia Vitória Savi,Daiani Ludwig,Thelma Alvim Veiga Ferreira,Paula Carolina Kava,Vanessa Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy |
author_facet |
Rampinelli,Viviane de Fátima Moura Aguillar Conceição,Daniele Santos,Beatriz Marra,Raquel Passos,Matheus Felipe Petersen,Rodolfo Vargas,José Viriato Coelho Galli-Terasawa,Lygia Vitória Savi,Daiani Ludwig,Thelma Alvim Veiga Ferreira,Paula Carolina Kava,Vanessa |
author_sort |
Rampinelli,Viviane de Fátima Moura Aguillar |
title |
Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy |
title_short |
Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy |
title_full |
Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy |
title_fullStr |
Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy |
title_full_unstemmed |
Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy |
title_sort |
paranaguá estuarine complex diatoms: morphology and molecular taxonomy |
description |
Abstract The Paranaguá Estuarine Complex (PEC) is one of Brazil's largest southwest Atlantic estuarine systems, possessing a rich microalgae diversity that remains to be fully explored. Therefore, due to the increasing interest in the microalgae biotechnological potential, this study isolated and identified diatoms found in the PEC. The diatoms were purified and analyzed with light and scanning electron microscopy for morphological identification, while DNA sequences were used for molecular identification. Although a diatoms rich diversity was obtained, only a few were viable after the cultivation period. The two best-selected strains were identified as belonging to two genera, Nitzschia and Navicula. The rbcL region was found to be the most informative for species identification. Morphological and molecular analyses allowed for the identification of species Nitzschia inconspicua and Navicula pseudoantonii, which was understood as the first report of N. pseudoantonii in the American continent. |
publisher |
Instituto de Tecnologia do Paraná - Tecpar |
publishDate |
2023 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100402 |
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