Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy

Abstract The Paranaguá Estuarine Complex (PEC) is one of Brazil's largest southwest Atlantic estuarine systems, possessing a rich microalgae diversity that remains to be fully explored. Therefore, due to the increasing interest in the microalgae biotechnological potential, this study isolated and identified diatoms found in the PEC. The diatoms were purified and analyzed with light and scanning electron microscopy for morphological identification, while DNA sequences were used for molecular identification. Although a diatoms rich diversity was obtained, only a few were viable after the cultivation period. The two best-selected strains were identified as belonging to two genera, Nitzschia and Navicula. The rbcL region was found to be the most informative for species identification. Morphological and molecular analyses allowed for the identification of species Nitzschia inconspicua and Navicula pseudoantonii, which was understood as the first report of N. pseudoantonii in the American continent.

Saved in:
Bibliographic Details
Main Authors: Rampinelli,Viviane de Fátima Moura Aguillar, Conceição,Daniele, Santos,Beatriz, Marra,Raquel, Passos,Matheus Felipe, Petersen,Rodolfo, Vargas,José Viriato Coelho, Galli-Terasawa,Lygia Vitória, Savi,Daiani, Ludwig,Thelma Alvim Veiga, Ferreira,Paula Carolina, Kava,Vanessa
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2023
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100402
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S1516-89132023000100402
record_format ojs
spelling oai:scielo:S1516-891320230001004022022-09-13Paranaguá Estuarine Complex Diatoms: Morphology and Molecular TaxonomyRampinelli,Viviane de Fátima Moura AguillarConceição,DanieleSantos,BeatrizMarra,RaquelPassos,Matheus FelipePetersen,RodolfoVargas,José Viriato CoelhoGalli-Terasawa,Lygia VitóriaSavi,DaianiLudwig,Thelma Alvim VeigaFerreira,Paula CarolinaKava,Vanessa bioprospection DNA-barcode rbcL Navicula pseudoantonii Nitzschia inconspicua Abstract The Paranaguá Estuarine Complex (PEC) is one of Brazil's largest southwest Atlantic estuarine systems, possessing a rich microalgae diversity that remains to be fully explored. Therefore, due to the increasing interest in the microalgae biotechnological potential, this study isolated and identified diatoms found in the PEC. The diatoms were purified and analyzed with light and scanning electron microscopy for morphological identification, while DNA sequences were used for molecular identification. Although a diatoms rich diversity was obtained, only a few were viable after the cultivation period. The two best-selected strains were identified as belonging to two genera, Nitzschia and Navicula. The rbcL region was found to be the most informative for species identification. Morphological and molecular analyses allowed for the identification of species Nitzschia inconspicua and Navicula pseudoantonii, which was understood as the first report of N. pseudoantonii in the American continent.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.66 20232023-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100402en10.1590/1678-4324-2023210523
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Rampinelli,Viviane de Fátima Moura Aguillar
Conceição,Daniele
Santos,Beatriz
Marra,Raquel
Passos,Matheus Felipe
Petersen,Rodolfo
Vargas,José Viriato Coelho
Galli-Terasawa,Lygia Vitória
Savi,Daiani
Ludwig,Thelma Alvim Veiga
Ferreira,Paula Carolina
Kava,Vanessa
spellingShingle Rampinelli,Viviane de Fátima Moura Aguillar
Conceição,Daniele
Santos,Beatriz
Marra,Raquel
Passos,Matheus Felipe
Petersen,Rodolfo
Vargas,José Viriato Coelho
Galli-Terasawa,Lygia Vitória
Savi,Daiani
Ludwig,Thelma Alvim Veiga
Ferreira,Paula Carolina
Kava,Vanessa
Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy
author_facet Rampinelli,Viviane de Fátima Moura Aguillar
Conceição,Daniele
Santos,Beatriz
Marra,Raquel
Passos,Matheus Felipe
Petersen,Rodolfo
Vargas,José Viriato Coelho
Galli-Terasawa,Lygia Vitória
Savi,Daiani
Ludwig,Thelma Alvim Veiga
Ferreira,Paula Carolina
Kava,Vanessa
author_sort Rampinelli,Viviane de Fátima Moura Aguillar
title Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy
title_short Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy
title_full Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy
title_fullStr Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy
title_full_unstemmed Paranaguá Estuarine Complex Diatoms: Morphology and Molecular Taxonomy
title_sort paranaguá estuarine complex diatoms: morphology and molecular taxonomy
description Abstract The Paranaguá Estuarine Complex (PEC) is one of Brazil's largest southwest Atlantic estuarine systems, possessing a rich microalgae diversity that remains to be fully explored. Therefore, due to the increasing interest in the microalgae biotechnological potential, this study isolated and identified diatoms found in the PEC. The diatoms were purified and analyzed with light and scanning electron microscopy for morphological identification, while DNA sequences were used for molecular identification. Although a diatoms rich diversity was obtained, only a few were viable after the cultivation period. The two best-selected strains were identified as belonging to two genera, Nitzschia and Navicula. The rbcL region was found to be the most informative for species identification. Morphological and molecular analyses allowed for the identification of species Nitzschia inconspicua and Navicula pseudoantonii, which was understood as the first report of N. pseudoantonii in the American continent.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132023000100402
work_keys_str_mv AT rampinellivivianedefatimamouraaguillar paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT conceicaodaniele paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT santosbeatriz paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT marraraquel paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT passosmatheusfelipe paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT petersenrodolfo paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT vargasjoseviriatocoelho paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT galliterasawalygiavitoria paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT savidaiani paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT ludwigthelmaalvimveiga paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT ferreirapaulacarolina paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
AT kavavanessa paranaguaestuarinecomplexdiatomsmorphologyandmoleculartaxonomy
_version_ 1756424094133256192