Possible Common Origin of B Chromosomes in Neotropical Fish (Siluriformes, Auchenipteridae) Reinforced by Repetitive DNA Mapping
Abstract The aim of this paper was to present the second case of B chromosomes in Auchenipteridae (Trachelyopterus sp.), and to test the hypothesis that the B chromosomes of this species and Parauchenipterus galeatus might have a common origin, since these two species have phylogenetic proximity. Both species have 58 chromosomes in the A complement, heterochromatin preferentially located at terminal region of the most of chromosomes, simple Ag-NORs located at the short arm of a subtelocentric pair, which was confirmed by hybridization with 18S rDNA, two submetacentric pairs carrying 5S rDNA sites, and presence of B chromosomes. The B chromosomes of the two species are small, metacentric, and almost totally heterochromatic, with variation of number intra and interindividual. In addition, for the first time in fish, the telomeric sequence [TTAGGG]n was dispersed along the B chromosomes (both species). The [GATA]n microsatellite were scattered in all chromosomes of the A complement and absent in the B chromosomes, in both species. These aspects confirm the phylogenetic proximity between the genus Parauchenipterus and Trachelyopterus, and they suggest the hypothesis that the B chromosomes of the two species might have common origin, previous to the diversification of these genera.
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Instituto de Tecnologia do Paraná - Tecpar
2021
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oai:scielo:S1516-891320210001004092021-05-11Possible Common Origin of B Chromosomes in Neotropical Fish (Siluriformes, Auchenipteridae) Reinforced by Repetitive DNA MappingLui,Roberto LaridondoTraldi,Josiane BaccarinBlanco,Daniel RodriguesMargarido,Vladimir PavanMariotto,SandraCentofante,LianoArtoni,Roberto FerreiraMoreira Filho,Orlando chromosome evolution [TTAGGG]n [GATA]n Parauchenipterus Trachelyopterus Abstract The aim of this paper was to present the second case of B chromosomes in Auchenipteridae (Trachelyopterus sp.), and to test the hypothesis that the B chromosomes of this species and Parauchenipterus galeatus might have a common origin, since these two species have phylogenetic proximity. Both species have 58 chromosomes in the A complement, heterochromatin preferentially located at terminal region of the most of chromosomes, simple Ag-NORs located at the short arm of a subtelocentric pair, which was confirmed by hybridization with 18S rDNA, two submetacentric pairs carrying 5S rDNA sites, and presence of B chromosomes. The B chromosomes of the two species are small, metacentric, and almost totally heterochromatic, with variation of number intra and interindividual. In addition, for the first time in fish, the telomeric sequence [TTAGGG]n was dispersed along the B chromosomes (both species). The [GATA]n microsatellite were scattered in all chromosomes of the A complement and absent in the B chromosomes, in both species. These aspects confirm the phylogenetic proximity between the genus Parauchenipterus and Trachelyopterus, and they suggest the hypothesis that the B chromosomes of the two species might have common origin, previous to the diversification of these genera.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.64 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100409en10.1590/1678-4324-2021190494 |
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Lui,Roberto Laridondo Traldi,Josiane Baccarin Blanco,Daniel Rodrigues Margarido,Vladimir Pavan Mariotto,Sandra Centofante,Liano Artoni,Roberto Ferreira Moreira Filho,Orlando |
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Lui,Roberto Laridondo Traldi,Josiane Baccarin Blanco,Daniel Rodrigues Margarido,Vladimir Pavan Mariotto,Sandra Centofante,Liano Artoni,Roberto Ferreira Moreira Filho,Orlando Possible Common Origin of B Chromosomes in Neotropical Fish (Siluriformes, Auchenipteridae) Reinforced by Repetitive DNA Mapping |
author_facet |
Lui,Roberto Laridondo Traldi,Josiane Baccarin Blanco,Daniel Rodrigues Margarido,Vladimir Pavan Mariotto,Sandra Centofante,Liano Artoni,Roberto Ferreira Moreira Filho,Orlando |
author_sort |
Lui,Roberto Laridondo |
title |
Possible Common Origin of B Chromosomes in Neotropical Fish (Siluriformes, Auchenipteridae) Reinforced by Repetitive DNA Mapping |
title_short |
Possible Common Origin of B Chromosomes in Neotropical Fish (Siluriformes, Auchenipteridae) Reinforced by Repetitive DNA Mapping |
title_full |
Possible Common Origin of B Chromosomes in Neotropical Fish (Siluriformes, Auchenipteridae) Reinforced by Repetitive DNA Mapping |
title_fullStr |
Possible Common Origin of B Chromosomes in Neotropical Fish (Siluriformes, Auchenipteridae) Reinforced by Repetitive DNA Mapping |
title_full_unstemmed |
Possible Common Origin of B Chromosomes in Neotropical Fish (Siluriformes, Auchenipteridae) Reinforced by Repetitive DNA Mapping |
title_sort |
possible common origin of b chromosomes in neotropical fish (siluriformes, auchenipteridae) reinforced by repetitive dna mapping |
description |
Abstract The aim of this paper was to present the second case of B chromosomes in Auchenipteridae (Trachelyopterus sp.), and to test the hypothesis that the B chromosomes of this species and Parauchenipterus galeatus might have a common origin, since these two species have phylogenetic proximity. Both species have 58 chromosomes in the A complement, heterochromatin preferentially located at terminal region of the most of chromosomes, simple Ag-NORs located at the short arm of a subtelocentric pair, which was confirmed by hybridization with 18S rDNA, two submetacentric pairs carrying 5S rDNA sites, and presence of B chromosomes. The B chromosomes of the two species are small, metacentric, and almost totally heterochromatic, with variation of number intra and interindividual. In addition, for the first time in fish, the telomeric sequence [TTAGGG]n was dispersed along the B chromosomes (both species). The [GATA]n microsatellite were scattered in all chromosomes of the A complement and absent in the B chromosomes, in both species. These aspects confirm the phylogenetic proximity between the genus Parauchenipterus and Trachelyopterus, and they suggest the hypothesis that the B chromosomes of the two species might have common origin, previous to the diversification of these genera. |
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Instituto de Tecnologia do Paraná - Tecpar |
publishDate |
2021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100409 |
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