Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina

Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina

Abstract The Apple Germplasm Bank (AGB) of Santa Catarina Agricultural Research and Rural Extension Company - Epagri, AGB-Epagri, is the largest of the genus Malus in Brazil. Twenty-eight main accessions of this bank were virus screened through DAS-ELISA, RT-PCR and IC-RT-PCR during two consecutive reproductive cycles, and each accession showed latent mixed infection by at least two species, among ASGV, ASPV and ACLSV. The combined use of diagnostic methods helped overcome inconsistencies commonly found in apple virus detection and was shown essential for the AGB-Epagri can be safely used as a source of genetic variability and for the exchange of virus-free propagative material.

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Main Authors: Manenti,Danielle Caroline, Hawerroth,Maraisa Crestani, Brancher,Thyana Lays, Schuh,Filipe Schmidt, Kvitschal,Marcus Vinicius, Nickel,Osmar, Souto,Eliezer Rodrigues de
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2020
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100215
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spelling oai:scielo:S1516-891320200001002152020-09-28Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa CatarinaManenti,Danielle CarolineHawerroth,Maraisa CrestaniBrancher,Thyana LaysSchuh,Filipe SchmidtKvitschal,Marcus ViniciusNickel,OsmarSouto,Eliezer Rodrigues de apple stem grooving virus (ASGV) apple stem pitting virus (ASPV) apple chlorotic leaf spot virus (ACLSV) Malus spp Abstract The Apple Germplasm Bank (AGB) of Santa Catarina Agricultural Research and Rural Extension Company - Epagri, AGB-Epagri, is the largest of the genus Malus in Brazil. Twenty-eight main accessions of this bank were virus screened through DAS-ELISA, RT-PCR and IC-RT-PCR during two consecutive reproductive cycles, and each accession showed latent mixed infection by at least two species, among ASGV, ASPV and ACLSV. The combined use of diagnostic methods helped overcome inconsistencies commonly found in apple virus detection and was shown essential for the AGB-Epagri can be safely used as a source of genetic variability and for the exchange of virus-free propagative material.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.63 20202020-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100215en10.1590/1678-4324-2020190272
institution SCIELO
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country Brasil
countrycode BR
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Manenti,Danielle Caroline
Hawerroth,Maraisa Crestani
Brancher,Thyana Lays
Schuh,Filipe Schmidt
Kvitschal,Marcus Vinicius
Nickel,Osmar
Souto,Eliezer Rodrigues de
spellingShingle Manenti,Danielle Caroline
Hawerroth,Maraisa Crestani
Brancher,Thyana Lays
Schuh,Filipe Schmidt
Kvitschal,Marcus Vinicius
Nickel,Osmar
Souto,Eliezer Rodrigues de
Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina
author_facet Manenti,Danielle Caroline
Hawerroth,Maraisa Crestani
Brancher,Thyana Lays
Schuh,Filipe Schmidt
Kvitschal,Marcus Vinicius
Nickel,Osmar
Souto,Eliezer Rodrigues de
author_sort Manenti,Danielle Caroline
title Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina
title_short Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina
title_full Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina
title_fullStr Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina
title_full_unstemmed Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina
title_sort serological and molecular detection of latent viruses in the apple germplasm bank of santa catarina
description Abstract The Apple Germplasm Bank (AGB) of Santa Catarina Agricultural Research and Rural Extension Company - Epagri, AGB-Epagri, is the largest of the genus Malus in Brazil. Twenty-eight main accessions of this bank were virus screened through DAS-ELISA, RT-PCR and IC-RT-PCR during two consecutive reproductive cycles, and each accession showed latent mixed infection by at least two species, among ASGV, ASPV and ACLSV. The combined use of diagnostic methods helped overcome inconsistencies commonly found in apple virus detection and was shown essential for the AGB-Epagri can be safely used as a source of genetic variability and for the exchange of virus-free propagative material.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100215
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