Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
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Instituto de Tecnologia do Paraná - Tecpar
2013
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oai:scielo:S1516-891320130006000122014-01-15Isolation and purification of bromelain from waste peel of pineapple for therapeutic applicationBresolin,Iara Rocha Antunes PereiraBresolin,Igor Tadeu LazzarottoSilveira,EdgarTambourgi,Elias BasileMazzola,Priscila Gava Bromelain pineapple peel downstream processing waste therapeutic applications enzymatic activity The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.56 n.6 20132013-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012en10.1590/S1516-89132013000600012 |
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Bresolin,Iara Rocha Antunes Pereira Bresolin,Igor Tadeu Lazzarotto Silveira,Edgar Tambourgi,Elias Basile Mazzola,Priscila Gava |
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Bresolin,Iara Rocha Antunes Pereira Bresolin,Igor Tadeu Lazzarotto Silveira,Edgar Tambourgi,Elias Basile Mazzola,Priscila Gava Isolation and purification of bromelain from waste peel of pineapple for therapeutic application |
| author_facet |
Bresolin,Iara Rocha Antunes Pereira Bresolin,Igor Tadeu Lazzarotto Silveira,Edgar Tambourgi,Elias Basile Mazzola,Priscila Gava |
| author_sort |
Bresolin,Iara Rocha Antunes Pereira |
| title |
Isolation and purification of bromelain from waste peel of pineapple for therapeutic application |
| title_short |
Isolation and purification of bromelain from waste peel of pineapple for therapeutic application |
| title_full |
Isolation and purification of bromelain from waste peel of pineapple for therapeutic application |
| title_fullStr |
Isolation and purification of bromelain from waste peel of pineapple for therapeutic application |
| title_full_unstemmed |
Isolation and purification of bromelain from waste peel of pineapple for therapeutic application |
| title_sort |
isolation and purification of bromelain from waste peel of pineapple for therapeutic application |
| description |
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity. |
| publisher |
Instituto de Tecnologia do Paraná - Tecpar |
| publishDate |
2013 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012 |
| work_keys_str_mv |
AT bresoliniararochaantunespereira isolationandpurificationofbromelainfromwastepeelofpineapplefortherapeuticapplication AT bresolinigortadeulazzarotto isolationandpurificationofbromelainfromwastepeelofpineapplefortherapeuticapplication AT silveiraedgar isolationandpurificationofbromelainfromwastepeelofpineapplefortherapeuticapplication AT tambourgieliasbasile isolationandpurificationofbromelainfromwastepeelofpineapplefortherapeuticapplication AT mazzolapriscilagava isolationandpurificationofbromelainfromwastepeelofpineapplefortherapeuticapplication |
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