Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus

Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus

Neonatal diarrhea is an important cause of economic losses for cattle farmers. The main viral etiologies of enteric diseases are group A rotaviruses (GARV) and the bovine coronavirus (BCoV). Although both viruses infect calves of the same age, the occurrence of mixed infections is still under studied. The present study describes the co-infection of BCoV and GARV in stool samples. Forty-four diarrheic fecal samples from calves up to 60 days old that had previously tested positive for GARV by SS-PAGE were analyzed using semi-nested PCR for BCoV. A product with 251 bp of the BCoV nucleoprotein gene was amplified in 15.9% (7/44) of the samples, demonstrating that co-infection is not an unusual event. These results reinforce the need for testing for both GARV and BCoV, even in fecal samples that previously tested positive for one virus.

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Main Authors: Barry,Aline Fernandes, Alfieri,Alice Fernandes, Stipp,Danilo Tancler, Alfieri,Amauri Alcindo
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2009
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700006
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spelling oai:scielo:S1516-891320090007000062010-02-11Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirusBarry,Aline FernandesAlfieri,Alice FernandesStipp,Danilo TanclerAlfieri,Amauri Alcindo calf diarrhea BCoV rotavirus SN-PCR co-infection Neonatal diarrhea is an important cause of economic losses for cattle farmers. The main viral etiologies of enteric diseases are group A rotaviruses (GARV) and the bovine coronavirus (BCoV). Although both viruses infect calves of the same age, the occurrence of mixed infections is still under studied. The present study describes the co-infection of BCoV and GARV in stool samples. Forty-four diarrheic fecal samples from calves up to 60 days old that had previously tested positive for GARV by SS-PAGE were analyzed using semi-nested PCR for BCoV. A product with 251 bp of the BCoV nucleoprotein gene was amplified in 15.9% (7/44) of the samples, demonstrating that co-infection is not an unusual event. These results reinforce the need for testing for both GARV and BCoV, even in fecal samples that previously tested positive for one virus.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.52 n.spe 20092009-11-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700006en10.1590/S1516-89132009000700006
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Barry,Aline Fernandes
Alfieri,Alice Fernandes
Stipp,Danilo Tancler
Alfieri,Amauri Alcindo
spellingShingle Barry,Aline Fernandes
Alfieri,Alice Fernandes
Stipp,Danilo Tancler
Alfieri,Amauri Alcindo
Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
author_facet Barry,Aline Fernandes
Alfieri,Alice Fernandes
Stipp,Danilo Tancler
Alfieri,Amauri Alcindo
author_sort Barry,Aline Fernandes
title Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
title_short Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
title_full Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
title_fullStr Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
title_full_unstemmed Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
title_sort bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
description Neonatal diarrhea is an important cause of economic losses for cattle farmers. The main viral etiologies of enteric diseases are group A rotaviruses (GARV) and the bovine coronavirus (BCoV). Although both viruses infect calves of the same age, the occurrence of mixed infections is still under studied. The present study describes the co-infection of BCoV and GARV in stool samples. Forty-four diarrheic fecal samples from calves up to 60 days old that had previously tested positive for GARV by SS-PAGE were analyzed using semi-nested PCR for BCoV. A product with 251 bp of the BCoV nucleoprotein gene was amplified in 15.9% (7/44) of the samples, demonstrating that co-infection is not an unusual event. These results reinforce the need for testing for both GARV and BCoV, even in fecal samples that previously tested positive for one virus.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700006
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AT alfierialicefernandes bovinecoronavirusdetectioninacollectionofdiarrheicstoolsamplespositiveforgroupabovinerotavirus
AT stippdanilotancler bovinecoronavirusdetectioninacollectionofdiarrheicstoolsamplespositiveforgroupabovinerotavirus
AT alfieriamaurialcindo bovinecoronavirusdetectioninacollectionofdiarrheicstoolsamplespositiveforgroupabovinerotavirus
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