An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species

An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species

An efficient and rapid DNA minipreparation modified method for frozen samples was developed for five tropical tree species: Copaifera langsdorffii, Hymenaea courbaril, Eugenia uniflora, Tabebuia roseo alba and Cariniana estrellensis. This procedure that dispenses the use of liquid nitrogen, phenol and the addition of proteinase K, is an adaptation of the CTAB-based DNA extraction method. The modifications included the use of PVP to eliminate the polyphenols, only one chloroform-isoamyl alcohol step and the addition of RNase immediately after extraction with chloroform. The yields of the DNA samples ranged from 25.7 to 42.1 µg from 100 mg leaf tissue. The DNA samples extracted by this method were successfully used for PCR (SSR and RAPD) analyses in these five and other twelve tropical tree species.

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Main Authors: Alzate-Marin,Ana Lilia, Guidugli,Marcela Corbo, Soriani,Hilda Hildebrand, Martinez,Carlos Alberto, Mestriner,Moacyr Antônio
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2009
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500020
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spelling oai:scielo:S1516-891320090005000202010-01-12An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree speciesAlzate-Marin,Ana LiliaGuidugli,Marcela CorboSoriani,Hilda HildebrandMartinez,Carlos AlbertoMestriner,Moacyr Antônio Copaifera langsdorffii Hymenaea courbaril Eugenia uniflora Tabebuia roseo alba Cariniana estrellensis DNA extraction An efficient and rapid DNA minipreparation modified method for frozen samples was developed for five tropical tree species: Copaifera langsdorffii, Hymenaea courbaril, Eugenia uniflora, Tabebuia roseo alba and Cariniana estrellensis. This procedure that dispenses the use of liquid nitrogen, phenol and the addition of proteinase K, is an adaptation of the CTAB-based DNA extraction method. The modifications included the use of PVP to eliminate the polyphenols, only one chloroform-isoamyl alcohol step and the addition of RNase immediately after extraction with chloroform. The yields of the DNA samples ranged from 25.7 to 42.1 µg from 100 mg leaf tissue. The DNA samples extracted by this method were successfully used for PCR (SSR and RAPD) analyses in these five and other twelve tropical tree species.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.52 n.5 20092009-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500020en10.1590/S1516-89132009000500020
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Alzate-Marin,Ana Lilia
Guidugli,Marcela Corbo
Soriani,Hilda Hildebrand
Martinez,Carlos Alberto
Mestriner,Moacyr Antônio
spellingShingle Alzate-Marin,Ana Lilia
Guidugli,Marcela Corbo
Soriani,Hilda Hildebrand
Martinez,Carlos Alberto
Mestriner,Moacyr Antônio
An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species
author_facet Alzate-Marin,Ana Lilia
Guidugli,Marcela Corbo
Soriani,Hilda Hildebrand
Martinez,Carlos Alberto
Mestriner,Moacyr Antônio
author_sort Alzate-Marin,Ana Lilia
title An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species
title_short An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species
title_full An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species
title_fullStr An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species
title_full_unstemmed An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species
title_sort efficient and rapid dna minipreparation procedure suitable for pcr/ssr and rapd analyses in tropical forest tree species
description An efficient and rapid DNA minipreparation modified method for frozen samples was developed for five tropical tree species: Copaifera langsdorffii, Hymenaea courbaril, Eugenia uniflora, Tabebuia roseo alba and Cariniana estrellensis. This procedure that dispenses the use of liquid nitrogen, phenol and the addition of proteinase K, is an adaptation of the CTAB-based DNA extraction method. The modifications included the use of PVP to eliminate the polyphenols, only one chloroform-isoamyl alcohol step and the addition of RNase immediately after extraction with chloroform. The yields of the DNA samples ranged from 25.7 to 42.1 µg from 100 mg leaf tissue. The DNA samples extracted by this method were successfully used for PCR (SSR and RAPD) analyses in these five and other twelve tropical tree species.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500020
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