Hydrolysis of whey lactose by immobilized β-Galactosidase

Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.

Bibliographic Details

Main Authors:	Mariotti,Marcela Panaro, Yamanaka,Hideko, Araujo,Angela Regina, Trevisan,Henrique Celso
Format:	Digital revista
Language:	English
Published:	Instituto de Tecnologia do Paraná - Tecpar 2008
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
Tags:	Add Tag No Tags, Be the first to tag this record!