Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae)
Microsporogenesis was analyzed in an interspecific hybrid between an artificially tetraploidized sexual accession of Brachiaria ruziziensis (2n=4x=36) and a natural apomictic tetraploid accession of B. decumbens. Syncytes involving a large number of cells were recorded in 15.4% of meiocytes. Meiosis progressed normally in syncytes during prophase I; in metaphase I, however, several nuclei were found fusioned, showing chromosome stickiness and several chromosome fragments. Meiosis was arrested in metaphase I and pycnotic nuclei and micronuclei were formed. Abnormal cytokinesis fractionated the syncyte into abnormal meiotic products that were covered by the pollen wall. Meiocytes in leptotene were recorded in all the slides prepared for both meiotic divisions, and abnormal "pollen grains" with well-developed pollen wall but containing leptotene nuclei were recorded in 9.18% of grains analyzed. These findings suggested that the meiocytes received the signal to enter meiosis but lacked the signal to proceed beyond leptotene. Despite the absence of the meiotic process, such cells were covered by pollen grain wall. Total pollen sterility resulted from these abnormalities combined with still others observed among meiocytes.
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Instituto de Tecnologia do Paraná - Tecpar
2007
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oai:scielo:S1516-891320070005000112007-11-23Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae)Mendes-Bonato,Andréa BeatrizPagliarini,Maria SuelyValle,Cacilda Borges do Brachiaria decumbens Brachiaria ruziziensis cell fusion interspecific hybrid meiosis arrest pollen sterilitiy Microsporogenesis was analyzed in an interspecific hybrid between an artificially tetraploidized sexual accession of Brachiaria ruziziensis (2n=4x=36) and a natural apomictic tetraploid accession of B. decumbens. Syncytes involving a large number of cells were recorded in 15.4% of meiocytes. Meiosis progressed normally in syncytes during prophase I; in metaphase I, however, several nuclei were found fusioned, showing chromosome stickiness and several chromosome fragments. Meiosis was arrested in metaphase I and pycnotic nuclei and micronuclei were formed. Abnormal cytokinesis fractionated the syncyte into abnormal meiotic products that were covered by the pollen wall. Meiocytes in leptotene were recorded in all the slides prepared for both meiotic divisions, and abnormal "pollen grains" with well-developed pollen wall but containing leptotene nuclei were recorded in 9.18% of grains analyzed. These findings suggested that the meiocytes received the signal to enter meiosis but lacked the signal to proceed beyond leptotene. Despite the absence of the meiotic process, such cells were covered by pollen grain wall. Total pollen sterility resulted from these abnormalities combined with still others observed among meiocytes.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.50 n.5 20072007-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500011en10.1590/S1516-89132007000500011 |
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Mendes-Bonato,Andréa Beatriz Pagliarini,Maria Suely Valle,Cacilda Borges do |
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Mendes-Bonato,Andréa Beatriz Pagliarini,Maria Suely Valle,Cacilda Borges do Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae) |
author_facet |
Mendes-Bonato,Andréa Beatriz Pagliarini,Maria Suely Valle,Cacilda Borges do |
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Mendes-Bonato,Andréa Beatriz |
title |
Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae) |
title_short |
Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae) |
title_full |
Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae) |
title_fullStr |
Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae) |
title_full_unstemmed |
Meiotic arrest compromises pollen fertility in an interspecific hybrid between Brachiaria ruziziensis x Brachiaria decumbens (Poaceae: paniceae) |
title_sort |
meiotic arrest compromises pollen fertility in an interspecific hybrid between brachiaria ruziziensis x brachiaria decumbens (poaceae: paniceae) |
description |
Microsporogenesis was analyzed in an interspecific hybrid between an artificially tetraploidized sexual accession of Brachiaria ruziziensis (2n=4x=36) and a natural apomictic tetraploid accession of B. decumbens. Syncytes involving a large number of cells were recorded in 15.4% of meiocytes. Meiosis progressed normally in syncytes during prophase I; in metaphase I, however, several nuclei were found fusioned, showing chromosome stickiness and several chromosome fragments. Meiosis was arrested in metaphase I and pycnotic nuclei and micronuclei were formed. Abnormal cytokinesis fractionated the syncyte into abnormal meiotic products that were covered by the pollen wall. Meiocytes in leptotene were recorded in all the slides prepared for both meiotic divisions, and abnormal "pollen grains" with well-developed pollen wall but containing leptotene nuclei were recorded in 9.18% of grains analyzed. These findings suggested that the meiocytes received the signal to enter meiosis but lacked the signal to proceed beyond leptotene. Despite the absence of the meiotic process, such cells were covered by pollen grain wall. Total pollen sterility resulted from these abnormalities combined with still others observed among meiocytes. |
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Instituto de Tecnologia do Paraná - Tecpar |
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2007 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500011 |
work_keys_str_mv |
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