Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.
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Instituto de Tecnologia do Paraná - Tecpar
2006
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oai:scielo:S1516-891320060005000032006-08-30Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase systemBezerra,Raquel PedrosaBorba,Fernanda Katharine Sousa LinsMoreira,Keila AparecidaLima-Filho,José LuisPorto,Ana Lúcia FigueiredoChaves,Adilson Castro Amylases aqueous two-phase systems Bacillus subtilis partition poly (ethylene glycol) Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.49 n.4 20062006-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003en10.1590/S1516-89132006000500003 |
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Bezerra,Raquel Pedrosa Borba,Fernanda Katharine Sousa Lins Moreira,Keila Aparecida Lima-Filho,José Luis Porto,Ana Lúcia Figueiredo Chaves,Adilson Castro |
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Bezerra,Raquel Pedrosa Borba,Fernanda Katharine Sousa Lins Moreira,Keila Aparecida Lima-Filho,José Luis Porto,Ana Lúcia Figueiredo Chaves,Adilson Castro Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system |
author_facet |
Bezerra,Raquel Pedrosa Borba,Fernanda Katharine Sousa Lins Moreira,Keila Aparecida Lima-Filho,José Luis Porto,Ana Lúcia Figueiredo Chaves,Adilson Castro |
author_sort |
Bezerra,Raquel Pedrosa |
title |
Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system |
title_short |
Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system |
title_full |
Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system |
title_fullStr |
Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system |
title_full_unstemmed |
Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system |
title_sort |
extraction of amylase from fermentation broth in poly (ethylene glycol) salt aqueous two-phase system |
description |
Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase. |
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Instituto de Tecnologia do Paraná - Tecpar |
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2006 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003 |
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