Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system

Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system

Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.

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Main Authors: Bezerra,Raquel Pedrosa, Borba,Fernanda Katharine Sousa Lins, Moreira,Keila Aparecida, Lima-Filho,José Luis, Porto,Ana Lúcia Figueiredo, Chaves,Adilson Castro
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2006
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003
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spelling oai:scielo:S1516-891320060005000032006-08-30Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase systemBezerra,Raquel PedrosaBorba,Fernanda Katharine Sousa LinsMoreira,Keila AparecidaLima-Filho,José LuisPorto,Ana Lúcia FigueiredoChaves,Adilson Castro Amylases aqueous two-phase systems Bacillus subtilis partition poly (ethylene glycol) Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.49 n.4 20062006-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003en10.1590/S1516-89132006000500003
institution SCIELO
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Bezerra,Raquel Pedrosa
Borba,Fernanda Katharine Sousa Lins
Moreira,Keila Aparecida
Lima-Filho,José Luis
Porto,Ana Lúcia Figueiredo
Chaves,Adilson Castro
spellingShingle Bezerra,Raquel Pedrosa
Borba,Fernanda Katharine Sousa Lins
Moreira,Keila Aparecida
Lima-Filho,José Luis
Porto,Ana Lúcia Figueiredo
Chaves,Adilson Castro
Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
author_facet Bezerra,Raquel Pedrosa
Borba,Fernanda Katharine Sousa Lins
Moreira,Keila Aparecida
Lima-Filho,José Luis
Porto,Ana Lúcia Figueiredo
Chaves,Adilson Castro
author_sort Bezerra,Raquel Pedrosa
title Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
title_short Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
title_full Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
title_fullStr Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
title_full_unstemmed Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
title_sort extraction of amylase from fermentation broth in poly (ethylene glycol) salt aqueous two-phase system
description Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003
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AT borbafernandakatharinesousalins extractionofamylasefromfermentationbrothinpolyethyleneglycolsaltaqueoustwophasesystem
AT moreirakeilaaparecida extractionofamylasefromfermentationbrothinpolyethyleneglycolsaltaqueoustwophasesystem
AT limafilhojoseluis extractionofamylasefromfermentationbrothinpolyethyleneglycolsaltaqueoustwophasesystem
AT portoanaluciafigueiredo extractionofamylasefromfermentationbrothinpolyethyleneglycolsaltaqueoustwophasesystem
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