Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)

A hundred adult of tilapia, Oreochromis niloticus, were inoculated intraperitoneally with 10(8) c.f.u. of Mycobacterium marinum. Other 100 speciemns were maintained without inoculation. Blood samples of six inoculated animals were collected at 01, 03, 07, 14, 21, 28, 35, 42, 49, 56 and 84 days after inoculation. Initially, six specimens were taken from the non inoculated group ("time zero"). The haematological parameters determined were: haematocrit (Hct), haemoglobin concentration (Hgb), total erythrocytes count (RBC), hematological indexes (MCV, MCH and MCHC), thrombocyte count (Tr), leukocyte count (WBC) and leukocyte differential count. There were no changes in Hct, Hgb, MCH and MCHC. However, the mean values for RBC and MCV decreased. The mean values for RBC and Hct of specimens from the "time zero" group were higher and the MCV and MCH lower than in inoculated animals. Occurred neutrophilia, and, at 72 h there was lymphocytosis and neutropenia. By the 49th day after bacterial inoculation, there was monocytosis.

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Main Authors: Ranzani-Paiva,Maria José Tavares, Ishikawa,Carlos Massatoshi, Eiras,Augusta Cocuzza das, Silveira,Vivian Risaffi da
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2004
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600014
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spelling oai:scielo:S1516-891320040006000142005-02-10Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)Ranzani-Paiva,Maria José TavaresIshikawa,Carlos MassatoshiEiras,Augusta Cocuzza dasSilveira,Vivian Risaffi da Mycobacterium marinum tilapia Oreochromis niloticus hematology A hundred adult of tilapia, Oreochromis niloticus, were inoculated intraperitoneally with 10(8) c.f.u. of Mycobacterium marinum. Other 100 speciemns were maintained without inoculation. Blood samples of six inoculated animals were collected at 01, 03, 07, 14, 21, 28, 35, 42, 49, 56 and 84 days after inoculation. Initially, six specimens were taken from the non inoculated group ("time zero"). The haematological parameters determined were: haematocrit (Hct), haemoglobin concentration (Hgb), total erythrocytes count (RBC), hematological indexes (MCV, MCH and MCHC), thrombocyte count (Tr), leukocyte count (WBC) and leukocyte differential count. There were no changes in Hct, Hgb, MCH and MCHC. However, the mean values for RBC and MCV decreased. The mean values for RBC and Hct of specimens from the "time zero" group were higher and the MCV and MCH lower than in inoculated animals. Occurred neutrophilia, and, at 72 h there was lymphocytosis and neutropenia. By the 49th day after bacterial inoculation, there was monocytosis.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.47 n.6 20042004-11-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600014en10.1590/S1516-89132004000600014
institution SCIELO
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Ranzani-Paiva,Maria José Tavares
Ishikawa,Carlos Massatoshi
Eiras,Augusta Cocuzza das
Silveira,Vivian Risaffi da
spellingShingle Ranzani-Paiva,Maria José Tavares
Ishikawa,Carlos Massatoshi
Eiras,Augusta Cocuzza das
Silveira,Vivian Risaffi da
Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)
author_facet Ranzani-Paiva,Maria José Tavares
Ishikawa,Carlos Massatoshi
Eiras,Augusta Cocuzza das
Silveira,Vivian Risaffi da
author_sort Ranzani-Paiva,Maria José Tavares
title Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)
title_short Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)
title_full Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)
title_fullStr Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)
title_full_unstemmed Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)
title_sort effects of an experimental challenge with mycobacterium marinum on the blood parameters of nile tilapia, oreochromis niloticus (linnaeus, 1757)
description A hundred adult of tilapia, Oreochromis niloticus, were inoculated intraperitoneally with 10(8) c.f.u. of Mycobacterium marinum. Other 100 speciemns were maintained without inoculation. Blood samples of six inoculated animals were collected at 01, 03, 07, 14, 21, 28, 35, 42, 49, 56 and 84 days after inoculation. Initially, six specimens were taken from the non inoculated group ("time zero"). The haematological parameters determined were: haematocrit (Hct), haemoglobin concentration (Hgb), total erythrocytes count (RBC), hematological indexes (MCV, MCH and MCHC), thrombocyte count (Tr), leukocyte count (WBC) and leukocyte differential count. There were no changes in Hct, Hgb, MCH and MCHC. However, the mean values for RBC and MCV decreased. The mean values for RBC and Hct of specimens from the "time zero" group were higher and the MCV and MCH lower than in inoculated animals. Occurred neutrophilia, and, at 72 h there was lymphocytosis and neutropenia. By the 49th day after bacterial inoculation, there was monocytosis.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600014
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