Chromatographic characterization of products isolated from chrome shavings

Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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Main Authors: Garcia,Edivaldo E, Kimura,Cláudia C.M, Martins,Ariovaldo C, Rocha,Gisele O, Nozaki,Jorge
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 1999
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300003
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spelling oai:scielo:S1516-891319990003000032011-06-01Chromatographic characterization of products isolated from chrome shavingsGarcia,Edivaldo EKimura,Cláudia C.MMartins,Ariovaldo CRocha,Gisele ONozaki,Jorge Chrome shavings enzyme chromatography protein chromium Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.42 n.3 19991999-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300003en10.1590/S1516-89131999000300003
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Garcia,Edivaldo E
Kimura,Cláudia C.M
Martins,Ariovaldo C
Rocha,Gisele O
Nozaki,Jorge
spellingShingle Garcia,Edivaldo E
Kimura,Cláudia C.M
Martins,Ariovaldo C
Rocha,Gisele O
Nozaki,Jorge
Chromatographic characterization of products isolated from chrome shavings
author_facet Garcia,Edivaldo E
Kimura,Cláudia C.M
Martins,Ariovaldo C
Rocha,Gisele O
Nozaki,Jorge
author_sort Garcia,Edivaldo E
title Chromatographic characterization of products isolated from chrome shavings
title_short Chromatographic characterization of products isolated from chrome shavings
title_full Chromatographic characterization of products isolated from chrome shavings
title_fullStr Chromatographic characterization of products isolated from chrome shavings
title_full_unstemmed Chromatographic characterization of products isolated from chrome shavings
title_sort chromatographic characterization of products isolated from chrome shavings
description Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 1999
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300003
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