Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle
ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle.
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Fundacao de Apoio a Ciência e Tecnologia Avicolas
2017
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oai:scielo:S1516-635X20170005000552017-05-18Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast MuscleGao,GWang,HZhao,XLi,QWang,CLi,JZhang,KZhong,HWang,Q Feeding conditions chicken FATP1 meat quality intramuscular fat ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle.info:eu-repo/semantics/openAccessFundacao de Apoio a Ciência e Tecnologia AvicolasBrazilian Journal of Poultry Science v.19 n.spe 20172017-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055en10.1590/1806-9061-2016-0367 |
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Gao,G Wang,H Zhao,X Li,Q Wang,C Li,J Zhang,K Zhong,H Wang,Q |
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Gao,G Wang,H Zhao,X Li,Q Wang,C Li,J Zhang,K Zhong,H Wang,Q Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
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Gao,G Wang,H Zhao,X Li,Q Wang,C Li,J Zhang,K Zhong,H Wang,Q |
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Gao,G |
title |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title_short |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title_full |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title_fullStr |
Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
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Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle |
title_sort |
effect of feeding conditions on the methylation status of fatp1 gene in chicken breast muscle |
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ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene. These results suggested that feeding conditions affect the methylation status and expression level of FATP1, thereby affecting the Intramuscular fat content in DNH and QJM chicken breast muscle. |
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Fundacao de Apoio a Ciência e Tecnologia Avicolas |
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2017 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055 |
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