In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens
The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes.
Main Authors: | Zimmermann,CEP, Machado,AK, Cadoná,FC, Jaques,JAS, Schlemmer,KB, Lautert,C, Cruz,IBM, Zanette,RA, Leal,DBR, Santurio,JM |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Fundacao de Apoio a Ciência e Tecnologia Avicolas
2014
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Evaluation of the effect of enzymatic hydrolyzate of the bursa of broiler chickens on cytotoxic activity and nonspecific immunity
by: Kolberg, N. A, et al.
Published: (2021) -
Efficacy of piperine in reducing the effects of aflatoxin intoxication in broiler chickens: a preliminary report
by: Cardoso,V.S., et al.
Published: (2011) -
Effect of three different anti-mycotoxin additives on broiler chickens exposed to aflatoxin B1
by: Oliveira,AA, et al.
Published: (2015) -
Natural feed additives for broiler chickens
by: Hayajneh,F.M.F.
Published: (2019) -
Adjustment of growth models in broiler chickens
by: Demuner,Leandro Félix, et al.
Published: (2017)