Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers
The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2) before two treatments: stimulation (6 Holstein and 8 Tabapuã), 500 mg of rbST (Boostin®) on D0 and 500 IU of eCG (Folligon) on D2; and control (6 Holstein and 8 Tabapuã), in which heifers received injections of the excipient. Heifers were aspirated on D4. Oocytes were subjected to a well established commercial in vitro embryo production protocol (Vitrogen®) and embryos were evaluated seven days after fertilization. There was an effect from the interaction of treatment and breed, so that hormonal stimulation increased antral follicle count (2-8 mm) in Tabapuã (29.9±2.6 to 41.4±2.6), but not in Holstein heifers (14.4±2.6 to 15.5±2.6). Tabapuã heifers had higher mean antral follicle count than Holsteins (35.6±1.8 vs. 15.0±2.1). The number of viable oocytes was not increased by stimulation in Tabapuã (from 4.7±1.0 to 5.2±1.1 in control and simulation, respectively) or in Holstein heifers (1.3±1.9 to 2.0±1.6 in control and simulation, respectively). There was no difference in the percentage of heifers with more than five viable oocytes in the group treated (33 vs 27%). The number of blastocysts was not affected by treatment (1.75 vs. 1.00 in hormonal stimulation and control, respectively). The increase in antral follicle count in the stimulated Tabapuã heifers did not reflect upon oocyte yield. The differential breed response to the hormonal treatment underscores the need for additional tests, especially for the Holstein breed, in order to enhance OPU efficiency.
Main Authors: | Morais,Hévea de, Spuri,Renata, Gonçalves,Tarcísio de Moraes, Carvalho,Rafaela Rodrigues de, Andrade,Renato Campos, Pinto,Tássia Louregiani Carvalho, Souza,José Camisão de |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Zootecnia
2012
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200004 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Prepartum administration of recombinant bovine somatotropin (rBST) on adaptation to subclinical ketosis of the ewes and performance of the lambs
by: Feijó,J.O., et al.
Published: (2015) -
The treatment of dairy buffalo with sustained release of rBST (Recombinant Bovine Somatotropin). 1- Effect on milk production and characteristics
by: Piccioli Cappelli, F. (Istituto di Zootecnica, Piacenza (Italy), et al.
Published: (1993) -
Effects of flunixin meglumine, recombinant bovine somatotropin and/or human chorionic gonadotropin on pregnancy rates in Nelore cows.
by: ROSSETTI, R. C., et al.
Published: (2011-12-29) -
Follicle-stimulating hormone to substitute equine chorionic gonadotropin in the synchronization of ovulation in Santa Inês ewes
by: Cardoso Neto,Bianor Matias, et al.
Published: (2012) -
Equine Chorionic Gonadotropin (eCG) improves bucks’ semen quality during the nonbreeding season
by: Beracochea, F., et al.
Published: (2018)