Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)

Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)

In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.

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Main Authors: Naskar,Soumen, Deb,Sitangsu M., Niranjan,Saket K., Kumar,Subodh, Sharma,Deepak, Sakaram,Durgam, Sharma,Arjava
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Genética 2012
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013
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spelling oai:scielo:S1415-475720120001000132012-03-28Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)Naskar,SoumenDeb,Sitangsu M.Niranjan,Saket K.Kumar,SubodhSharma,DeepakSakaram,DurgamSharma,Arjava water buffalo cDNA DRB MHC phylogeny In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.35 n.1 20122012-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013en10.1590/S1415-47572012005000013
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Naskar,Soumen
Deb,Sitangsu M.
Niranjan,Saket K.
Kumar,Subodh
Sharma,Deepak
Sakaram,Durgam
Sharma,Arjava
spellingShingle Naskar,Soumen
Deb,Sitangsu M.
Niranjan,Saket K.
Kumar,Subodh
Sharma,Deepak
Sakaram,Durgam
Sharma,Arjava
Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
author_facet Naskar,Soumen
Deb,Sitangsu M.
Niranjan,Saket K.
Kumar,Subodh
Sharma,Deepak
Sakaram,Durgam
Sharma,Arjava
author_sort Naskar,Soumen
title Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_short Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_full Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_fullStr Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_full_unstemmed Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
title_sort molecular characterization of mhc-drb cdna in water buffalo (bubalus bubalis)
description In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.
publisher Sociedade Brasileira de Genética
publishDate 2012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013
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