Development of microsatellite markers for identifying Brazilian Coffea arabica varieties
Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffea liberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of 2.5. Based on the banding patterns generated by polymorphic SSR loci, the set of twenty-five coffee varieties were clustered into two main groups, one composed of only Brazilian varieties, and the other of interspecific hybrids, with a few Brazilians. Color mutants could not be separated. Clustering was in accordance with material genealogy thereby revealing high similarity.
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Sociedade Brasileira de Genética
2010
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oai:scielo:S1415-475720100003000222010-08-18Development of microsatellite markers for identifying Brazilian Coffea arabica varietiesVieira,Elisa S.N.Von Pinho,Édila V. de R.Carvalho,Maria G.G.Esselink,Danny G.Vosman,Ben SSR coffee genetic similarity molecular marker Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffea liberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of 2.5. Based on the banding patterns generated by polymorphic SSR loci, the set of twenty-five coffee varieties were clustered into two main groups, one composed of only Brazilian varieties, and the other of interspecific hybrids, with a few Brazilians. Color mutants could not be separated. Clustering was in accordance with material genealogy thereby revealing high similarity.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.33 n.3 20102010-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300022en10.1590/S1415-47572010005000055 |
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Vieira,Elisa S.N. Von Pinho,Édila V. de R. Carvalho,Maria G.G. Esselink,Danny G. Vosman,Ben |
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Vieira,Elisa S.N. Von Pinho,Édila V. de R. Carvalho,Maria G.G. Esselink,Danny G. Vosman,Ben Development of microsatellite markers for identifying Brazilian Coffea arabica varieties |
author_facet |
Vieira,Elisa S.N. Von Pinho,Édila V. de R. Carvalho,Maria G.G. Esselink,Danny G. Vosman,Ben |
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Vieira,Elisa S.N. |
title |
Development of microsatellite markers for identifying Brazilian Coffea arabica varieties |
title_short |
Development of microsatellite markers for identifying Brazilian Coffea arabica varieties |
title_full |
Development of microsatellite markers for identifying Brazilian Coffea arabica varieties |
title_fullStr |
Development of microsatellite markers for identifying Brazilian Coffea arabica varieties |
title_full_unstemmed |
Development of microsatellite markers for identifying Brazilian Coffea arabica varieties |
title_sort |
development of microsatellite markers for identifying brazilian coffea arabica varieties |
description |
Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffea liberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of 2.5. Based on the banding patterns generated by polymorphic SSR loci, the set of twenty-five coffee varieties were clustered into two main groups, one composed of only Brazilian varieties, and the other of interspecific hybrids, with a few Brazilians. Color mutants could not be separated. Clustering was in accordance with material genealogy thereby revealing high similarity. |
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Sociedade Brasileira de Genética |
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2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300022 |
work_keys_str_mv |
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