SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity

Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly moderate bootstrap support. These major clusters corresponded with the photoperiod response and place of origin. The results indicate that the photoperiod insensitive genotypes, 11/2/1939 (EC 325097) and MACS 330 would be better choice for broadening the genetic base of soybean for this trait.

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Main Authors: Singh,Ram K., Bhatia,Virendra S., Bhat,K.V., Mohapatra,Trilochan, Singh,Nagendra K., Bansal,Kailash C., Koundal,K.R.
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Genética 2010
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200019
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spelling oai:scielo:S1415-475720100002000192010-06-14SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivitySingh,Ram K.Bhatia,Virendra S.Bhat,K.V.Mohapatra,TrilochanSingh,Nagendra K.Bansal,Kailash C.Koundal,K.R. photoperiod response SSR AFLP genetic diversity soybean Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly moderate bootstrap support. These major clusters corresponded with the photoperiod response and place of origin. The results indicate that the photoperiod insensitive genotypes, 11/2/1939 (EC 325097) and MACS 330 would be better choice for broadening the genetic base of soybean for this trait.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.33 n.2 20102010-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200019en10.1590/S1415-47572010005000024
institution SCIELO
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Singh,Ram K.
Bhatia,Virendra S.
Bhat,K.V.
Mohapatra,Trilochan
Singh,Nagendra K.
Bansal,Kailash C.
Koundal,K.R.
spellingShingle Singh,Ram K.
Bhatia,Virendra S.
Bhat,K.V.
Mohapatra,Trilochan
Singh,Nagendra K.
Bansal,Kailash C.
Koundal,K.R.
SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
author_facet Singh,Ram K.
Bhatia,Virendra S.
Bhat,K.V.
Mohapatra,Trilochan
Singh,Nagendra K.
Bansal,Kailash C.
Koundal,K.R.
author_sort Singh,Ram K.
title SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
title_short SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
title_full SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
title_fullStr SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
title_full_unstemmed SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
title_sort ssr and aflp based genetic diversity of soybean germplasm differing in photoperiod sensitivity
description Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly moderate bootstrap support. These major clusters corresponded with the photoperiod response and place of origin. The results indicate that the photoperiod insensitive genotypes, 11/2/1939 (EC 325097) and MACS 330 would be better choice for broadening the genetic base of soybean for this trait.
publisher Sociedade Brasileira de Genética
publishDate 2010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200019
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