Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA

Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA

Phakopsora pachyrhizi has dispersed globally and brought severe economic losses to soybean growers. The fungus has been established in Brazil since 2002 and is found nationwide. To gather information on the temporal and spatial patterns of genetic variation in P. pachyrhizi , we sequenced the nuclear internal transcribed spacer regions (ITS1 and ITS2). Total genomic DNA was extracted using either lyophilized urediniospores or lesions removed from infected leaves sampled from 26 soybean fields in Brazil and one field in South Africa. Cloning prior to sequencing was necessary because direct sequencing of PCR amplicons gave partially unreadable electrophoretograms with peak displacements suggestive of multiple sequences with length polymorphism. Sequences were determined from four clones per field. ITS sequences from African or Asian isolates available from the GenBank were included in the analyses. Independent sequence alignments of the ITS1 and ITS2 datasets identified 27 and 19 ribotypes, respectively. Molecular phylogeographic analyses revealed that ribotypes of widespread distribution in Brazil displayed characteristics of ancestrality and were shared with Africa and Asia, while ribotypes of rare occurrence in Brazil were indigenous. The results suggest P. pachyrhizi found in Brazil as originating from multiple, independent long-distance dispersal events.

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Main Authors: Freire,Maíra C. M., Oliveira,Luiz O. de, Almeida,Álvaro M. R. de, Schuster,Ivan, Moreira,Maurilio A., Liebenberg,Merion M., Mienie,Charlotte M. S.
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Genética 2008
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500020
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spelling oai:scielo:S1415-475720080005000202008-11-19Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNAFreire,Maíra C. M.Oliveira,Luiz O. deAlmeida,Álvaro M. R. deSchuster,IvanMoreira,Maurilio A.Liebenberg,Merion M.Mienie,Charlotte M. S. Phakopsora pachyrhizi diversity internal transcribed spacer Asian soybean rust phylogeography Phakopsora pachyrhizi has dispersed globally and brought severe economic losses to soybean growers. The fungus has been established in Brazil since 2002 and is found nationwide. To gather information on the temporal and spatial patterns of genetic variation in P. pachyrhizi , we sequenced the nuclear internal transcribed spacer regions (ITS1 and ITS2). Total genomic DNA was extracted using either lyophilized urediniospores or lesions removed from infected leaves sampled from 26 soybean fields in Brazil and one field in South Africa. Cloning prior to sequencing was necessary because direct sequencing of PCR amplicons gave partially unreadable electrophoretograms with peak displacements suggestive of multiple sequences with length polymorphism. Sequences were determined from four clones per field. ITS sequences from African or Asian isolates available from the GenBank were included in the analyses. Independent sequence alignments of the ITS1 and ITS2 datasets identified 27 and 19 ribotypes, respectively. Molecular phylogeographic analyses revealed that ribotypes of widespread distribution in Brazil displayed characteristics of ancestrality and were shared with Africa and Asia, while ribotypes of rare occurrence in Brazil were indigenous. The results suggest P. pachyrhizi found in Brazil as originating from multiple, independent long-distance dispersal events. info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.31 n.4 20082008-12-01text/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500020en10.1590/S1415-47572008005000026
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
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region America del Sur
libraryname SciELO
language English
format Digital
author Freire,Maíra C. M.
Oliveira,Luiz O. de
Almeida,Álvaro M. R. de
Schuster,Ivan
Moreira,Maurilio A.
Liebenberg,Merion M.
Mienie,Charlotte M. S.
spellingShingle Freire,Maíra C. M.
Oliveira,Luiz O. de
Almeida,Álvaro M. R. de
Schuster,Ivan
Moreira,Maurilio A.
Liebenberg,Merion M.
Mienie,Charlotte M. S.
Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA
author_facet Freire,Maíra C. M.
Oliveira,Luiz O. de
Almeida,Álvaro M. R. de
Schuster,Ivan
Moreira,Maurilio A.
Liebenberg,Merion M.
Mienie,Charlotte M. S.
author_sort Freire,Maíra C. M.
title Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA
title_short Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA
title_full Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA
title_fullStr Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA
title_full_unstemmed Evolutionary history of Phakopsora pachyrhizi (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA
title_sort evolutionary history of phakopsora pachyrhizi (the asian soybean rust) in brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal dna
description Phakopsora pachyrhizi has dispersed globally and brought severe economic losses to soybean growers. The fungus has been established in Brazil since 2002 and is found nationwide. To gather information on the temporal and spatial patterns of genetic variation in P. pachyrhizi , we sequenced the nuclear internal transcribed spacer regions (ITS1 and ITS2). Total genomic DNA was extracted using either lyophilized urediniospores or lesions removed from infected leaves sampled from 26 soybean fields in Brazil and one field in South Africa. Cloning prior to sequencing was necessary because direct sequencing of PCR amplicons gave partially unreadable electrophoretograms with peak displacements suggestive of multiple sequences with length polymorphism. Sequences were determined from four clones per field. ITS sequences from African or Asian isolates available from the GenBank were included in the analyses. Independent sequence alignments of the ITS1 and ITS2 datasets identified 27 and 19 ribotypes, respectively. Molecular phylogeographic analyses revealed that ribotypes of widespread distribution in Brazil displayed characteristics of ancestrality and were shared with Africa and Asia, while ribotypes of rare occurrence in Brazil were indigenous. The results suggest P. pachyrhizi found in Brazil as originating from multiple, independent long-distance dispersal events.
publisher Sociedade Brasileira de Genética
publishDate 2008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500020
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