DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.
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Sociedade Brasileira de Genética
2008
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oai:scielo:S1415-475720080004000052008-08-18DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patientsLima,Patrícia Santos PereiraMolffeta,Greice AndreotiAraujo,Amélia Góes deZago,Marco AntônioSilva Jr.,Wilson Araújo da CpG island DNA methylation CDKN2B The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.31 n.3 20082008-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005en10.1590/S1415-47572008000400005 |
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Lima,Patrícia Santos Pereira Molffeta,Greice Andreoti Araujo,Amélia Góes de Zago,Marco Antônio Silva Jr.,Wilson Araújo da |
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Lima,Patrícia Santos Pereira Molffeta,Greice Andreoti Araujo,Amélia Góes de Zago,Marco Antônio Silva Jr.,Wilson Araújo da DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients |
author_facet |
Lima,Patrícia Santos Pereira Molffeta,Greice Andreoti Araujo,Amélia Góes de Zago,Marco Antônio Silva Jr.,Wilson Araújo da |
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Lima,Patrícia Santos Pereira |
title |
DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients |
title_short |
DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients |
title_full |
DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients |
title_fullStr |
DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients |
title_full_unstemmed |
DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients |
title_sort |
dna methylation analysis of the tumor suppressor gene cdkn2b in brazilian leukemia patients |
description |
The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients. |
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Sociedade Brasileira de Genética |
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2008 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005 |
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