The pgm locus and pigmentation phenotype in Yersinia pestis
The pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures on Congo red agar (CRA) plates. Strain P. CE 882 was shown to be highly stable while strains P. Exu 340 and P. Peru 375 dissociated into several phenotypes, PCR analysis showing evidence of changes in the pgm locus of the derived cultures. Strains P. Exu 340 and P. Peru 375 produced previously unreported cultures positive for the pesticin/yersiniabactin outer membrane receptor (psn+) but negative for the iron-regulated protein (irp2-), suggesting the occurrence of rearrangements in this chromosomal region and either a sequential loss or the loss of separated segments. These results provide evidence that besides deletion en bloc, specific rearrangements are also involved in the deletion events for that locus.
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Sociedade Brasileira de Genética
2006
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oai:scielo:S1415-475720060001000242006-03-10The pgm locus and pigmentation phenotype in Yersinia pestisLeal-Balbino,Tereza CristinaLeal,Nilma CintraNascimento,Mirna Gisele Medeiros doOliveira,Maria Betânia Melo deBalbino,Valdir de QueirozAlmeida,Alzira Maria Paiva de Y. pestis high pathogenicity island pgm psn The pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures on Congo red agar (CRA) plates. Strain P. CE 882 was shown to be highly stable while strains P. Exu 340 and P. Peru 375 dissociated into several phenotypes, PCR analysis showing evidence of changes in the pgm locus of the derived cultures. Strains P. Exu 340 and P. Peru 375 produced previously unreported cultures positive for the pesticin/yersiniabactin outer membrane receptor (psn+) but negative for the iron-regulated protein (irp2-), suggesting the occurrence of rearrangements in this chromosomal region and either a sequential loss or the loss of separated segments. These results provide evidence that besides deletion en bloc, specific rearrangements are also involved in the deletion events for that locus.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.29 n.1 20062006-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100024en10.1590/S1415-47572006000100024 |
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Leal-Balbino,Tereza Cristina Leal,Nilma Cintra Nascimento,Mirna Gisele Medeiros do Oliveira,Maria Betânia Melo de Balbino,Valdir de Queiroz Almeida,Alzira Maria Paiva de |
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Leal-Balbino,Tereza Cristina Leal,Nilma Cintra Nascimento,Mirna Gisele Medeiros do Oliveira,Maria Betânia Melo de Balbino,Valdir de Queiroz Almeida,Alzira Maria Paiva de The pgm locus and pigmentation phenotype in Yersinia pestis |
author_facet |
Leal-Balbino,Tereza Cristina Leal,Nilma Cintra Nascimento,Mirna Gisele Medeiros do Oliveira,Maria Betânia Melo de Balbino,Valdir de Queiroz Almeida,Alzira Maria Paiva de |
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Leal-Balbino,Tereza Cristina |
title |
The pgm locus and pigmentation phenotype in Yersinia pestis |
title_short |
The pgm locus and pigmentation phenotype in Yersinia pestis |
title_full |
The pgm locus and pigmentation phenotype in Yersinia pestis |
title_fullStr |
The pgm locus and pigmentation phenotype in Yersinia pestis |
title_full_unstemmed |
The pgm locus and pigmentation phenotype in Yersinia pestis |
title_sort |
pgm locus and pigmentation phenotype in yersinia pestis |
description |
The pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures on Congo red agar (CRA) plates. Strain P. CE 882 was shown to be highly stable while strains P. Exu 340 and P. Peru 375 dissociated into several phenotypes, PCR analysis showing evidence of changes in the pgm locus of the derived cultures. Strains P. Exu 340 and P. Peru 375 produced previously unreported cultures positive for the pesticin/yersiniabactin outer membrane receptor (psn+) but negative for the iron-regulated protein (irp2-), suggesting the occurrence of rearrangements in this chromosomal region and either a sequential loss or the loss of separated segments. These results provide evidence that besides deletion en bloc, specific rearrangements are also involved in the deletion events for that locus. |
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Sociedade Brasileira de Genética |
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2006 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100024 |
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