Pathogenesis of rabies virus by ERA and PV strains administered orally in hamsters (M. auratus)

Hamsters orally inoculated with ERA and PV strains of rabies virus were sacrificed at 24, 48, 72 hours, 21 and 30 days after inoculation. Brain fragments were examined by Fluorescent Antibody test (FAT) and heminested PCR (hn-PCR). Fragments from stomach, blood, heart, and lung were examined only by hn-PCR. Sera of other hamsters, similarly inoculated, obtained at 30th day after inoculation were submitted to mouse neutralization test. The hamsters were challenged intracerebrally with CVS strain with 10(2.7)mouse LD50/0.03mL, 45 days after inoculation. Brains examined by FAT were negative. The hn-PCR detected the presence of rabies virus RNA in the lung of one animal inoculated with ERA, and in the brain, stomach, blood, and lung of PV-infected animals. The orally inoculated virus was capable to infect and replicate in several organs and tissues; however, none of the challenged hamsters did survive after challenge.

Bibliographic Details

Main Authors:	Alves,Luciane Martins, Soares,Rodrigo Martins, Cortez,Adriana, Richtzenhain,Leonardo José, Ito,Fumio Nonma
Format:	Digital revista
Language:	English
Published:	Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo 2003
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000100010
Tags:	Add Tag No Tags, Be the first to tag this record!