Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies
ABSTRACTThe antifungal activity of some statins against different fungal species has been reported. Thus, at the first moment, the in vitro antifungal activity of simvastatin, atorvastatin and pravastatin was tested againstCandida spp. and Cryptococcus spp. Then, in a second approach, considering that the best results were obtained for simvastatin, this drug was evaluated in combination with antifungal drugs against planktonic growth and tested against biofilms ofCandida spp. and Cryptococcus spp. Drug susceptibility testing was performed using the microdilution broth method, as described by the Clinical and Laboratory Standards Institute. The interaction between simvastatin and antifungals against planktonic cells was analyzed by calculating the fractional inhibitory concentration index. Regarding biofilm susceptibility, simvastatin was tested against growing biofilm and mature biofilm of one strain of each tested yeast species. Simvastatin showed inhibitory effect against Candida spp. andCryptococcus spp. with minimum inhibitory concentration values ranging from 15.6 to 1000 mg L-1 and from 62.5 to 1000 mg L-1, respectively. The combination of simvastatin with itraconazole and fluconazole showed synergism against Candidaspp. and Cryptococcus spp., while the combination of simvastatin with amphotericin B was synergistic only againstCryptococcus spp. Concerning the biofilm assays, simvastatin was able to inhibit both growing biofilm and mature biofilm ofCandida spp. and Cryptococcus spp. The present study showed that simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcus species.
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Brazilian Society of Infectious Diseases
2015
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oai:scielo:S1413-867020150005004592015-11-06Simvastatin inhibits planktonic cells and biofilms ofCandida and CryptococcusspeciesBrilhante,Raimunda Sâmia NogueiraCaetano,Erica PachecoOliveira,Jonathas Sales deCastelo-Branco,Débora de Souza Collares MaiaSouza,Elizabeth Ribeiro YokobatakeAlencar,Lucas Pereira deCordeiro,Rossana de AguiarBandeira,Tereza de Jesus Pinheiro GomesSidrim,José Júlio CostaRocha,Marcos Fábio Gadelha Candida Cryptococcus Simvastatin Antifungal activity Biofilm ABSTRACTThe antifungal activity of some statins against different fungal species has been reported. Thus, at the first moment, the in vitro antifungal activity of simvastatin, atorvastatin and pravastatin was tested againstCandida spp. and Cryptococcus spp. Then, in a second approach, considering that the best results were obtained for simvastatin, this drug was evaluated in combination with antifungal drugs against planktonic growth and tested against biofilms ofCandida spp. and Cryptococcus spp. Drug susceptibility testing was performed using the microdilution broth method, as described by the Clinical and Laboratory Standards Institute. The interaction between simvastatin and antifungals against planktonic cells was analyzed by calculating the fractional inhibitory concentration index. Regarding biofilm susceptibility, simvastatin was tested against growing biofilm and mature biofilm of one strain of each tested yeast species. Simvastatin showed inhibitory effect against Candida spp. andCryptococcus spp. with minimum inhibitory concentration values ranging from 15.6 to 1000 mg L-1 and from 62.5 to 1000 mg L-1, respectively. The combination of simvastatin with itraconazole and fluconazole showed synergism against Candidaspp. and Cryptococcus spp., while the combination of simvastatin with amphotericin B was synergistic only againstCryptococcus spp. Concerning the biofilm assays, simvastatin was able to inhibit both growing biofilm and mature biofilm ofCandida spp. and Cryptococcus spp. The present study showed that simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcus species.info:eu-repo/semantics/openAccessBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious Diseases v.19 n.5 20152015-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500459en10.1016/j.bjid.2015.06.001 |
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Brilhante,Raimunda Sâmia Nogueira Caetano,Erica Pacheco Oliveira,Jonathas Sales de Castelo-Branco,Débora de Souza Collares Maia Souza,Elizabeth Ribeiro Yokobatake Alencar,Lucas Pereira de Cordeiro,Rossana de Aguiar Bandeira,Tereza de Jesus Pinheiro Gomes Sidrim,José Júlio Costa Rocha,Marcos Fábio Gadelha |
| spellingShingle |
Brilhante,Raimunda Sâmia Nogueira Caetano,Erica Pacheco Oliveira,Jonathas Sales de Castelo-Branco,Débora de Souza Collares Maia Souza,Elizabeth Ribeiro Yokobatake Alencar,Lucas Pereira de Cordeiro,Rossana de Aguiar Bandeira,Tereza de Jesus Pinheiro Gomes Sidrim,José Júlio Costa Rocha,Marcos Fábio Gadelha Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies |
| author_facet |
Brilhante,Raimunda Sâmia Nogueira Caetano,Erica Pacheco Oliveira,Jonathas Sales de Castelo-Branco,Débora de Souza Collares Maia Souza,Elizabeth Ribeiro Yokobatake Alencar,Lucas Pereira de Cordeiro,Rossana de Aguiar Bandeira,Tereza de Jesus Pinheiro Gomes Sidrim,José Júlio Costa Rocha,Marcos Fábio Gadelha |
| author_sort |
Brilhante,Raimunda Sâmia Nogueira |
| title |
Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies |
| title_short |
Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies |
| title_full |
Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies |
| title_fullStr |
Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies |
| title_full_unstemmed |
Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies |
| title_sort |
simvastatin inhibits planktonic cells and biofilms ofcandida and cryptococcusspecies |
| description |
ABSTRACTThe antifungal activity of some statins against different fungal species has been reported. Thus, at the first moment, the in vitro antifungal activity of simvastatin, atorvastatin and pravastatin was tested againstCandida spp. and Cryptococcus spp. Then, in a second approach, considering that the best results were obtained for simvastatin, this drug was evaluated in combination with antifungal drugs against planktonic growth and tested against biofilms ofCandida spp. and Cryptococcus spp. Drug susceptibility testing was performed using the microdilution broth method, as described by the Clinical and Laboratory Standards Institute. The interaction between simvastatin and antifungals against planktonic cells was analyzed by calculating the fractional inhibitory concentration index. Regarding biofilm susceptibility, simvastatin was tested against growing biofilm and mature biofilm of one strain of each tested yeast species. Simvastatin showed inhibitory effect against Candida spp. andCryptococcus spp. with minimum inhibitory concentration values ranging from 15.6 to 1000 mg L-1 and from 62.5 to 1000 mg L-1, respectively. The combination of simvastatin with itraconazole and fluconazole showed synergism against Candidaspp. and Cryptococcus spp., while the combination of simvastatin with amphotericin B was synergistic only againstCryptococcus spp. Concerning the biofilm assays, simvastatin was able to inhibit both growing biofilm and mature biofilm ofCandida spp. and Cryptococcus spp. The present study showed that simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcus species. |
| publisher |
Brazilian Society of Infectious Diseases |
| publishDate |
2015 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500459 |
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