Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum

Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum

OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. MATERIALS AND METHODS: Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes expression of treated L. infantum were studied. RESULTS: Miltefosine led to dose-dependent death of L. infantumwith features compatible with apoptosis. Over expression of metacaspase and PARP was seen 6 hr after treatment. CONCLUSIONS: Our study showed that miltefosine exerts cytotoxic effect on L. infantum via an apoptotic-related mechanism.

Saved in:
Bibliographic Details
Main Authors: Khademvatan,Shahram, Gharavi,Mohammad Javad, Saki,Jasem
Format: Digital revista
Language:English
Published: Brazilian Society of Infectious Diseases 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500005
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S1413-86702011000500005
record_format ojs
spelling oai:scielo:S1413-867020110005000052011-10-17Miltefosine induces metacaspase and PARP genes expression in Leishmania infantumKhademvatan,ShahramGharavi,Mohammad JavadSaki,Jasem Leishmania infantum apoptosis gene expression OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. MATERIALS AND METHODS: Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes expression of treated L. infantum were studied. RESULTS: Miltefosine led to dose-dependent death of L. infantumwith features compatible with apoptosis. Over expression of metacaspase and PARP was seen 6 hr after treatment. CONCLUSIONS: Our study showed that miltefosine exerts cytotoxic effect on L. infantum via an apoptotic-related mechanism.info:eu-repo/semantics/openAccessBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious Diseases v.15 n.5 20112011-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500005en10.1590/S1413-86702011000500005
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Khademvatan,Shahram
Gharavi,Mohammad Javad
Saki,Jasem
spellingShingle Khademvatan,Shahram
Gharavi,Mohammad Javad
Saki,Jasem
Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
author_facet Khademvatan,Shahram
Gharavi,Mohammad Javad
Saki,Jasem
author_sort Khademvatan,Shahram
title Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
title_short Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
title_full Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
title_fullStr Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
title_full_unstemmed Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
title_sort miltefosine induces metacaspase and parp genes expression in leishmania infantum
description OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. MATERIALS AND METHODS: Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes expression of treated L. infantum were studied. RESULTS: Miltefosine led to dose-dependent death of L. infantumwith features compatible with apoptosis. Over expression of metacaspase and PARP was seen 6 hr after treatment. CONCLUSIONS: Our study showed that miltefosine exerts cytotoxic effect on L. infantum via an apoptotic-related mechanism.
publisher Brazilian Society of Infectious Diseases
publishDate 2011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500005
work_keys_str_mv AT khademvatanshahram miltefosineinducesmetacaspaseandparpgenesexpressioninleishmaniainfantum
AT gharavimohammadjavad miltefosineinducesmetacaspaseandparpgenesexpressioninleishmaniainfantum
AT sakijasem miltefosineinducesmetacaspaseandparpgenesexpressioninleishmaniainfantum
_version_ 1756416433520115712

Similar Items