Enrichment methodology to increase the positivity of cultures from body fluids
Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.
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Brazilian Society of Infectious Diseases
2006
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oai:scielo:S1413-867020060006000022007-03-30Enrichment methodology to increase the positivity of cultures from body fluidsDaur,Alessandra ValleKlimak Jr.,FranciscoCogo,Laura LúciaBotão,Gislene DiógenesMonteiro,Cristina Leise BastosDalla Costa,Libera Maria Body fluids etiological agents enrichment Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.info:eu-repo/semantics/openAccessBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious Diseases v.10 n.6 20062006-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600002en10.1590/S1413-86702006000600002 |
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Daur,Alessandra Valle Klimak Jr.,Francisco Cogo,Laura Lúcia Botão,Gislene Diógenes Monteiro,Cristina Leise Bastos Dalla Costa,Libera Maria |
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Daur,Alessandra Valle Klimak Jr.,Francisco Cogo,Laura Lúcia Botão,Gislene Diógenes Monteiro,Cristina Leise Bastos Dalla Costa,Libera Maria Enrichment methodology to increase the positivity of cultures from body fluids |
| author_facet |
Daur,Alessandra Valle Klimak Jr.,Francisco Cogo,Laura Lúcia Botão,Gislene Diógenes Monteiro,Cristina Leise Bastos Dalla Costa,Libera Maria |
| author_sort |
Daur,Alessandra Valle |
| title |
Enrichment methodology to increase the positivity of cultures from body fluids |
| title_short |
Enrichment methodology to increase the positivity of cultures from body fluids |
| title_full |
Enrichment methodology to increase the positivity of cultures from body fluids |
| title_fullStr |
Enrichment methodology to increase the positivity of cultures from body fluids |
| title_full_unstemmed |
Enrichment methodology to increase the positivity of cultures from body fluids |
| title_sort |
enrichment methodology to increase the positivity of cultures from body fluids |
| description |
Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents. |
| publisher |
Brazilian Society of Infectious Diseases |
| publishDate |
2006 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600002 |
| work_keys_str_mv |
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| _version_ |
1756416345586532352 |