Characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in Mexico
Abstract The objective of this study was to determine the relationships between four acute phase proteins (APP), and the pathological effects of porcine epidemic diarrhea virus (PEDV) in piglets and sows. Virus detection was done in laboratory (PCR method) with feces samples. Blood samples were obtained from lactating sows and piglets that survived the first day of life according to the presence or absence of characteristic enteric signs (ES) of the disease: vomiting and diarrhea. (I) Neonatal piglets without ES (n=7), (II) Neonatal piglets with ES (n=8), (III) Sows without ES (n=6), and (IV) Sows with ES (n=6). To determine the APP concentration in blood, serum amyloid A (SAA) and haptoglobin (HAP) were determined by a non species specific commercial ELISA assay. C-reactive protein (CRP) and PIGMAP serum levels were quantified by monoclonal antibody sandwich ELISA assay. Finally, no significant differences (P>0.05) were found in serum concentrations of different APP between piglets and sows with and without ES. Piglet survival is linked by the degree of dehydration during diarrhea; PEDV causes ES quickly and severely before of the induction of pro inflammatory cytokines, but this pathological synthesis still remains unclear.
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Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Zaragoza
2018
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oai:scielo:S1405-888X20180003211012020-11-27Characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in MexicoHernández-Trujillo,EleinBolaños-López,DanBeltrán-Figueroa,RolandoSarmiento-Silva,Rosa ElenaJuárez-López,NoéTrujillo-Ortega,María Elena porcine epidemic diarrhea virus acute phase proteins neonatal porcine mortality diagnosis Abstract The objective of this study was to determine the relationships between four acute phase proteins (APP), and the pathological effects of porcine epidemic diarrhea virus (PEDV) in piglets and sows. Virus detection was done in laboratory (PCR method) with feces samples. Blood samples were obtained from lactating sows and piglets that survived the first day of life according to the presence or absence of characteristic enteric signs (ES) of the disease: vomiting and diarrhea. (I) Neonatal piglets without ES (n=7), (II) Neonatal piglets with ES (n=8), (III) Sows without ES (n=6), and (IV) Sows with ES (n=6). To determine the APP concentration in blood, serum amyloid A (SAA) and haptoglobin (HAP) were determined by a non species specific commercial ELISA assay. C-reactive protein (CRP) and PIGMAP serum levels were quantified by monoclonal antibody sandwich ELISA assay. Finally, no significant differences (P>0.05) were found in serum concentrations of different APP between piglets and sows with and without ES. Piglet survival is linked by the degree of dehydration during diarrhea; PEDV causes ES quickly and severely before of the induction of pro inflammatory cytokines, but this pathological synthesis still remains unclear.info:eu-repo/semantics/openAccessUniversidad Nacional Autónoma de México, Facultad de Estudios Superiores ZaragozaTIP. Revista especializada en ciencias químico-biológicas v.21 suppl.1 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-888X2018000321101en10.22201/fesz.23958723e.2019.1.140 |
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México |
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MX |
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| author |
Hernández-Trujillo,Elein Bolaños-López,Dan Beltrán-Figueroa,Rolando Sarmiento-Silva,Rosa Elena Juárez-López,Noé Trujillo-Ortega,María Elena |
| spellingShingle |
Hernández-Trujillo,Elein Bolaños-López,Dan Beltrán-Figueroa,Rolando Sarmiento-Silva,Rosa Elena Juárez-López,Noé Trujillo-Ortega,María Elena Characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in Mexico |
| author_facet |
Hernández-Trujillo,Elein Bolaños-López,Dan Beltrán-Figueroa,Rolando Sarmiento-Silva,Rosa Elena Juárez-López,Noé Trujillo-Ortega,María Elena |
| author_sort |
Hernández-Trujillo,Elein |
| title |
Characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in Mexico |
| title_short |
Characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in Mexico |
| title_full |
Characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in Mexico |
| title_fullStr |
Characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in Mexico |
| title_full_unstemmed |
Characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in Mexico |
| title_sort |
characterization of acute phase proteins associated with a porcine epidemic diarrhea virus outbreak in suckling piglets and sows in mexico |
| description |
Abstract The objective of this study was to determine the relationships between four acute phase proteins (APP), and the pathological effects of porcine epidemic diarrhea virus (PEDV) in piglets and sows. Virus detection was done in laboratory (PCR method) with feces samples. Blood samples were obtained from lactating sows and piglets that survived the first day of life according to the presence or absence of characteristic enteric signs (ES) of the disease: vomiting and diarrhea. (I) Neonatal piglets without ES (n=7), (II) Neonatal piglets with ES (n=8), (III) Sows without ES (n=6), and (IV) Sows with ES (n=6). To determine the APP concentration in blood, serum amyloid A (SAA) and haptoglobin (HAP) were determined by a non species specific commercial ELISA assay. C-reactive protein (CRP) and PIGMAP serum levels were quantified by monoclonal antibody sandwich ELISA assay. Finally, no significant differences (P>0.05) were found in serum concentrations of different APP between piglets and sows with and without ES. Piglet survival is linked by the degree of dehydration during diarrhea; PEDV causes ES quickly and severely before of the induction of pro inflammatory cytokines, but this pathological synthesis still remains unclear. |
| publisher |
Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Zaragoza |
| publishDate |
2018 |
| url |
http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-888X2018000321101 |
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