Ethanol production potential of Saccharomyces fragilis IZ 275 using cheese whey powder solution
The use of residues from dairy industry is interesting because of the ease of acquisition and a relatively low cost. The objective of this study was to analyze ethanol production by the Saccharomyces fragilis varying the concentration of cheese whey powder (CWP) solution, initial pH and inoculum concentration using a factorial design technique; besides, semi-batch operations to add lactose was investigated. Statistical analysis was performed by ANOVA (p≤0.05). The concentrations of CWP solution and initial pH were significant in the fermentation medium for ethanol production. The optimum conditions were CWP solution 15 %, pH 5.0, inoculum concentration 5 % and a fermentation time of 18 h, and ethanol production reached 7.6 % (v/v). Additionaly, semi-batch operations performed to add lactose also modified the ethanol yield (10.67 % v/v). This would be the first time that a high ethanol production rate was obtained from S. fragilis using cheese whey powder solution and a factorial design technique.
Main Authors: | , , , , |
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Format: | Digital revista |
Language: | English |
Published: |
Colegio de Postgraduados
2015
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Online Access: | http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000300005 |
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Summary: | The use of residues from dairy industry is interesting because of the ease of acquisition and a relatively low cost. The objective of this study was to analyze ethanol production by the Saccharomyces fragilis varying the concentration of cheese whey powder (CWP) solution, initial pH and inoculum concentration using a factorial design technique; besides, semi-batch operations to add lactose was investigated. Statistical analysis was performed by ANOVA (p≤0.05). The concentrations of CWP solution and initial pH were significant in the fermentation medium for ethanol production. The optimum conditions were CWP solution 15 %, pH 5.0, inoculum concentration 5 % and a fermentation time of 18 h, and ethanol production reached 7.6 % (v/v). Additionaly, semi-batch operations performed to add lactose also modified the ethanol yield (10.67 % v/v). This would be the first time that a high ethanol production rate was obtained from S. fragilis using cheese whey powder solution and a factorial design technique. |
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