Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation
Background: Interstitial cells of Cajal (ICC) are required for normal motility in the gastrointestinal tract. Depletion of ICC has been associated with diabetic gastroenteropathy. Aim: To determine the effect of quercertin supplementation on anoctamin-1 (Ano1) immunoreactive ICC in the myenteric region (ICC-MY) and deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats. Methods: Thirty-two 90-day-old male Wistar rats were distributed into the following groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). Diabetes was induced by streptozotocin injection. After 120 days, preparations of the jejunal muscular and submucosal layers were immunostained for Ano1 to visualize ICC. Evaluation of the immunofluorescence intensity as well as density of ICC was performed. Results: The density of ICC-MY was 46% lower in group D compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After quercertin treatment, the densities of ICC-MY were significantly higher in the DQ group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin in normoglycemic animals (CQ) compared with group C did not significantly change the ICC density (p > 0.05). Conclusions: In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action.
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Sociedad Española de Patología Digestiva
2017
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oai:scielo:S1130-010820170003000052017-07-05Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementationVieira-Frez,Flávia CristinaMartins-Colombo-Perles,Juliana VanessaRobert-Linden,DavidGibbons,Simon JamesAmilcar-Martins,HeberAlmeida-Brito-Romualdo,Déborade-Souza,Sara RaquelDaion-Piovezana-Bossolani,GleisonNelisis Zanoni,Jacqueline Antioxidants Diabetes mellitus Interstitial cells of Cajal Quercetin Background: Interstitial cells of Cajal (ICC) are required for normal motility in the gastrointestinal tract. Depletion of ICC has been associated with diabetic gastroenteropathy. Aim: To determine the effect of quercertin supplementation on anoctamin-1 (Ano1) immunoreactive ICC in the myenteric region (ICC-MY) and deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats. Methods: Thirty-two 90-day-old male Wistar rats were distributed into the following groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). Diabetes was induced by streptozotocin injection. After 120 days, preparations of the jejunal muscular and submucosal layers were immunostained for Ano1 to visualize ICC. Evaluation of the immunofluorescence intensity as well as density of ICC was performed. Results: The density of ICC-MY was 46% lower in group D compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After quercertin treatment, the densities of ICC-MY were significantly higher in the DQ group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin in normoglycemic animals (CQ) compared with group C did not significantly change the ICC density (p > 0.05). Conclusions: In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action.Sociedad Española de Patología DigestivaRevista Española de Enfermedades Digestivas v.109 n.3 20172017-03-01journal articletext/htmlhttp://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082017000300005es |
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Vieira-Frez,Flávia Cristina Martins-Colombo-Perles,Juliana Vanessa Robert-Linden,David Gibbons,Simon James Amilcar-Martins,Heber Almeida-Brito-Romualdo,Débora de-Souza,Sara Raquel Daion-Piovezana-Bossolani,Gleison Nelisis Zanoni,Jacqueline |
| spellingShingle |
Vieira-Frez,Flávia Cristina Martins-Colombo-Perles,Juliana Vanessa Robert-Linden,David Gibbons,Simon James Amilcar-Martins,Heber Almeida-Brito-Romualdo,Débora de-Souza,Sara Raquel Daion-Piovezana-Bossolani,Gleison Nelisis Zanoni,Jacqueline Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation |
| author_facet |
Vieira-Frez,Flávia Cristina Martins-Colombo-Perles,Juliana Vanessa Robert-Linden,David Gibbons,Simon James Amilcar-Martins,Heber Almeida-Brito-Romualdo,Débora de-Souza,Sara Raquel Daion-Piovezana-Bossolani,Gleison Nelisis Zanoni,Jacqueline |
| author_sort |
Vieira-Frez,Flávia Cristina |
| title |
Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation |
| title_short |
Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation |
| title_full |
Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation |
| title_fullStr |
Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation |
| title_full_unstemmed |
Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation |
| title_sort |
restoration of density of interstitial cells of cajal in the jejunum of diabetic rats after quercetin supplementation |
| description |
Background: Interstitial cells of Cajal (ICC) are required for normal motility in the gastrointestinal tract. Depletion of ICC has been associated with diabetic gastroenteropathy. Aim: To determine the effect of quercertin supplementation on anoctamin-1 (Ano1) immunoreactive ICC in the myenteric region (ICC-MY) and deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats. Methods: Thirty-two 90-day-old male Wistar rats were distributed into the following groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). Diabetes was induced by streptozotocin injection. After 120 days, preparations of the jejunal muscular and submucosal layers were immunostained for Ano1 to visualize ICC. Evaluation of the immunofluorescence intensity as well as density of ICC was performed. Results: The density of ICC-MY was 46% lower in group D compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After quercertin treatment, the densities of ICC-MY were significantly higher in the DQ group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin in normoglycemic animals (CQ) compared with group C did not significantly change the ICC density (p > 0.05). Conclusions: In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action. |
| publisher |
Sociedad Española de Patología Digestiva |
| publishDate |
2017 |
| url |
http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082017000300005 |
| work_keys_str_mv |
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