Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence

Objective: the role of telomerase activity and telomere length in the adenoma-carcinoma sequence of colon carcinogenesis has not been well established. The objective of this study was to determine telomerase activity and telomere length patterns in patients with adenomatous polyps either associated or not with colorectal cancer, as well as the role of telomeric instability in the adenoma-carcinoma sequence. Patients and methods: we included in the study 14 patients who underwent surgery for colorectal cancer and/or polyps. In 6 of these patients fresh samples of tumor tissue, polyps, and normal mucosa were obtained; in the 8 remaining cases, we collected only polyps and normal mucosa. We used the fluorescent-telomeric repeat amplification protocol assay (TRAP-F) to determine telomerase activity and telomere length using Southern-blot testing. Results: telomerase activity was detected in 86% of polyps and 50% of associated normal mucosa. Mean telomerase activity in polyp tissue was 5.85; in the normal mucosa it was 0.58 TPG. Mean telomere length was 6.78 Kbp and 7.78, respectively. Polyps in patients without synchronous cancer had a telomerase activity that was significantly higher (9.4) than in those with cancer (1.1). Conclusions: telomerase activity increases in the colorectal adenoma-carcinoma sequence, concurrently with a decrease in telomere length. The presence of synchronous cancer modifies telomerase activity in polyps.

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Main Authors: Valls Bautista,C., Piñol Felis,C., Reñe Espinet,J. M., Buenestado García,J., Viñas Salas,J.
Format: Digital revista
Language:English
Published: Sociedad Española de Patología Digestiva 2009
Online Access:http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082009000300004
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spelling oai:scielo:S1130-010820090003000042009-08-25Telomerase activity and telomere length in the colorectal polyp-carcinoma sequenceValls Bautista,C.Piñol Felis,C.Reñe Espinet,J. M.Buenestado García,J.Viñas Salas,J. Telomerase activity Telomere length Colorectal Carcinoma Polyp Objective: the role of telomerase activity and telomere length in the adenoma-carcinoma sequence of colon carcinogenesis has not been well established. The objective of this study was to determine telomerase activity and telomere length patterns in patients with adenomatous polyps either associated or not with colorectal cancer, as well as the role of telomeric instability in the adenoma-carcinoma sequence. Patients and methods: we included in the study 14 patients who underwent surgery for colorectal cancer and/or polyps. In 6 of these patients fresh samples of tumor tissue, polyps, and normal mucosa were obtained; in the 8 remaining cases, we collected only polyps and normal mucosa. We used the fluorescent-telomeric repeat amplification protocol assay (TRAP-F) to determine telomerase activity and telomere length using Southern-blot testing. Results: telomerase activity was detected in 86% of polyps and 50% of associated normal mucosa. Mean telomerase activity in polyp tissue was 5.85; in the normal mucosa it was 0.58 TPG. Mean telomere length was 6.78 Kbp and 7.78, respectively. Polyps in patients without synchronous cancer had a telomerase activity that was significantly higher (9.4) than in those with cancer (1.1). Conclusions: telomerase activity increases in the colorectal adenoma-carcinoma sequence, concurrently with a decrease in telomere length. The presence of synchronous cancer modifies telomerase activity in polyps.Sociedad Española de Patología DigestivaRevista Española de Enfermedades Digestivas v.101 n.3 20092009-03-01journal articletext/htmlhttp://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082009000300004en
institution SCIELO
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country España
countrycode ES
component Revista
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databasecode rev-scielo-es
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region Europa del Sur
libraryname SciELO
language English
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author Valls Bautista,C.
Piñol Felis,C.
Reñe Espinet,J. M.
Buenestado García,J.
Viñas Salas,J.
spellingShingle Valls Bautista,C.
Piñol Felis,C.
Reñe Espinet,J. M.
Buenestado García,J.
Viñas Salas,J.
Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
author_facet Valls Bautista,C.
Piñol Felis,C.
Reñe Espinet,J. M.
Buenestado García,J.
Viñas Salas,J.
author_sort Valls Bautista,C.
title Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
title_short Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
title_full Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
title_fullStr Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
title_full_unstemmed Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
title_sort telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
description Objective: the role of telomerase activity and telomere length in the adenoma-carcinoma sequence of colon carcinogenesis has not been well established. The objective of this study was to determine telomerase activity and telomere length patterns in patients with adenomatous polyps either associated or not with colorectal cancer, as well as the role of telomeric instability in the adenoma-carcinoma sequence. Patients and methods: we included in the study 14 patients who underwent surgery for colorectal cancer and/or polyps. In 6 of these patients fresh samples of tumor tissue, polyps, and normal mucosa were obtained; in the 8 remaining cases, we collected only polyps and normal mucosa. We used the fluorescent-telomeric repeat amplification protocol assay (TRAP-F) to determine telomerase activity and telomere length using Southern-blot testing. Results: telomerase activity was detected in 86% of polyps and 50% of associated normal mucosa. Mean telomerase activity in polyp tissue was 5.85; in the normal mucosa it was 0.58 TPG. Mean telomere length was 6.78 Kbp and 7.78, respectively. Polyps in patients without synchronous cancer had a telomerase activity that was significantly higher (9.4) than in those with cancer (1.1). Conclusions: telomerase activity increases in the colorectal adenoma-carcinoma sequence, concurrently with a decrease in telomere length. The presence of synchronous cancer modifies telomerase activity in polyps.
publisher Sociedad Española de Patología Digestiva
publishDate 2009
url http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082009000300004
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