Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence
Objective: the role of telomerase activity and telomere length in the adenoma-carcinoma sequence of colon carcinogenesis has not been well established. The objective of this study was to determine telomerase activity and telomere length patterns in patients with adenomatous polyps either associated or not with colorectal cancer, as well as the role of telomeric instability in the adenoma-carcinoma sequence. Patients and methods: we included in the study 14 patients who underwent surgery for colorectal cancer and/or polyps. In 6 of these patients fresh samples of tumor tissue, polyps, and normal mucosa were obtained; in the 8 remaining cases, we collected only polyps and normal mucosa. We used the fluorescent-telomeric repeat amplification protocol assay (TRAP-F) to determine telomerase activity and telomere length using Southern-blot testing. Results: telomerase activity was detected in 86% of polyps and 50% of associated normal mucosa. Mean telomerase activity in polyp tissue was 5.85; in the normal mucosa it was 0.58 TPG. Mean telomere length was 6.78 Kbp and 7.78, respectively. Polyps in patients without synchronous cancer had a telomerase activity that was significantly higher (9.4) than in those with cancer (1.1). Conclusions: telomerase activity increases in the colorectal adenoma-carcinoma sequence, concurrently with a decrease in telomere length. The presence of synchronous cancer modifies telomerase activity in polyps.
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Sociedad Española de Patología Digestiva
2009
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oai:scielo:S1130-010820090003000042009-08-25Telomerase activity and telomere length in the colorectal polyp-carcinoma sequenceValls Bautista,C.Piñol Felis,C.Reñe Espinet,J. M.Buenestado García,J.Viñas Salas,J. Telomerase activity Telomere length Colorectal Carcinoma Polyp Objective: the role of telomerase activity and telomere length in the adenoma-carcinoma sequence of colon carcinogenesis has not been well established. The objective of this study was to determine telomerase activity and telomere length patterns in patients with adenomatous polyps either associated or not with colorectal cancer, as well as the role of telomeric instability in the adenoma-carcinoma sequence. Patients and methods: we included in the study 14 patients who underwent surgery for colorectal cancer and/or polyps. In 6 of these patients fresh samples of tumor tissue, polyps, and normal mucosa were obtained; in the 8 remaining cases, we collected only polyps and normal mucosa. We used the fluorescent-telomeric repeat amplification protocol assay (TRAP-F) to determine telomerase activity and telomere length using Southern-blot testing. Results: telomerase activity was detected in 86% of polyps and 50% of associated normal mucosa. Mean telomerase activity in polyp tissue was 5.85; in the normal mucosa it was 0.58 TPG. Mean telomere length was 6.78 Kbp and 7.78, respectively. Polyps in patients without synchronous cancer had a telomerase activity that was significantly higher (9.4) than in those with cancer (1.1). Conclusions: telomerase activity increases in the colorectal adenoma-carcinoma sequence, concurrently with a decrease in telomere length. The presence of synchronous cancer modifies telomerase activity in polyps.Sociedad Española de Patología DigestivaRevista Española de Enfermedades Digestivas v.101 n.3 20092009-03-01journal articletext/htmlhttp://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082009000300004en |
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Valls Bautista,C. Piñol Felis,C. Reñe Espinet,J. M. Buenestado García,J. Viñas Salas,J. |
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Valls Bautista,C. Piñol Felis,C. Reñe Espinet,J. M. Buenestado García,J. Viñas Salas,J. Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence |
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Valls Bautista,C. Piñol Felis,C. Reñe Espinet,J. M. Buenestado García,J. Viñas Salas,J. |
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Valls Bautista,C. |
title |
Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence |
title_short |
Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence |
title_full |
Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence |
title_fullStr |
Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence |
title_full_unstemmed |
Telomerase activity and telomere length in the colorectal polyp-carcinoma sequence |
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telomerase activity and telomere length in the colorectal polyp-carcinoma sequence |
description |
Objective: the role of telomerase activity and telomere length in the adenoma-carcinoma sequence of colon carcinogenesis has not been well established. The objective of this study was to determine telomerase activity and telomere length patterns in patients with adenomatous polyps either associated or not with colorectal cancer, as well as the role of telomeric instability in the adenoma-carcinoma sequence. Patients and methods: we included in the study 14 patients who underwent surgery for colorectal cancer and/or polyps. In 6 of these patients fresh samples of tumor tissue, polyps, and normal mucosa were obtained; in the 8 remaining cases, we collected only polyps and normal mucosa. We used the fluorescent-telomeric repeat amplification protocol assay (TRAP-F) to determine telomerase activity and telomere length using Southern-blot testing. Results: telomerase activity was detected in 86% of polyps and 50% of associated normal mucosa. Mean telomerase activity in polyp tissue was 5.85; in the normal mucosa it was 0.58 TPG. Mean telomere length was 6.78 Kbp and 7.78, respectively. Polyps in patients without synchronous cancer had a telomerase activity that was significantly higher (9.4) than in those with cancer (1.1). Conclusions: telomerase activity increases in the colorectal adenoma-carcinoma sequence, concurrently with a decrease in telomere length. The presence of synchronous cancer modifies telomerase activity in polyps. |
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Sociedad Española de Patología Digestiva |
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2009 |
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http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082009000300004 |
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