Polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitis
Background and objectives: interleukin-10 (IL-10) has a key role in regulating mucosal inflammation in inflammatory bowel disease. In our population of Spanish ulcerative colitis (UC) patients, we have previously demostrated that two polymorphisms (IL-10.G14 microsatellite allele and homozygous for the -1082G alelle (guanine at position -1082)) in the IL-10 gene were susceptibility markers for disease. No data exist regarding the relationship of these IL-10 polymorphims with phenotypic subpopulations in UC. Therefore, this study sought to examine the contribution of IL-10 polymorphims to phenotypical variability in UC. Material and methods: a cohort of 215 Spanish unrelated patients with UC recruited in a single center was studied. All patients were rigorously phenotyped and followed for at least 3 years (mean time: 11.8 years). The clinical phenotype was established before genotyping. Genotyping was performed using polymerase chain reaction (PCR) assays. Results: patiens with UC included 129 (60%) men and 89 (40%) women. Mean age at diagnosis was 38 years, with a range of 8-83. Disease extent included 127 (59.1%) left-side patients and 88 (40.9%) extensive patients. Neither UC phenotype variable was associated with the presence of susceptibility polymorphims (10G14 microsatellite and -1082G alelle). Conclusions: in Madrid's Spanish population of UC patients, the carrying of the ILG14 microsatellite or -1082G polymorphism in the IL-10 gene was not associated with phenotype of disease.
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Sociedad Española de Patología Digestiva
2006
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oai:scielo:S1130-010820060002000042009-07-14Polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitisMendoza,J. L.Urcelay,E.Lana,R.Martínez,A.Taxonera,C.Concha,E. G. de laDíaz-Rubio,M. Ulcerative colitis Interleukin-10 gene Phenotype Inflammatory bowel disease Background and objectives: interleukin-10 (IL-10) has a key role in regulating mucosal inflammation in inflammatory bowel disease. In our population of Spanish ulcerative colitis (UC) patients, we have previously demostrated that two polymorphisms (IL-10.G14 microsatellite allele and homozygous for the -1082G alelle (guanine at position -1082)) in the IL-10 gene were susceptibility markers for disease. No data exist regarding the relationship of these IL-10 polymorphims with phenotypic subpopulations in UC. Therefore, this study sought to examine the contribution of IL-10 polymorphims to phenotypical variability in UC. Material and methods: a cohort of 215 Spanish unrelated patients with UC recruited in a single center was studied. All patients were rigorously phenotyped and followed for at least 3 years (mean time: 11.8 years). The clinical phenotype was established before genotyping. Genotyping was performed using polymerase chain reaction (PCR) assays. Results: patiens with UC included 129 (60%) men and 89 (40%) women. Mean age at diagnosis was 38 years, with a range of 8-83. Disease extent included 127 (59.1%) left-side patients and 88 (40.9%) extensive patients. Neither UC phenotype variable was associated with the presence of susceptibility polymorphims (10G14 microsatellite and -1082G alelle). Conclusions: in Madrid's Spanish population of UC patients, the carrying of the ILG14 microsatellite or -1082G polymorphism in the IL-10 gene was not associated with phenotype of disease.Sociedad Española de Patología DigestivaRevista Española de Enfermedades Digestivas v.98 n.2 20062006-02-01journal articletext/htmlhttp://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082006000200004en |
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Mendoza,J. L. Urcelay,E. Lana,R. Martínez,A. Taxonera,C. Concha,E. G. de la Díaz-Rubio,M. |
spellingShingle |
Mendoza,J. L. Urcelay,E. Lana,R. Martínez,A. Taxonera,C. Concha,E. G. de la Díaz-Rubio,M. Polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitis |
author_facet |
Mendoza,J. L. Urcelay,E. Lana,R. Martínez,A. Taxonera,C. Concha,E. G. de la Díaz-Rubio,M. |
author_sort |
Mendoza,J. L. |
title |
Polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitis |
title_short |
Polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitis |
title_full |
Polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitis |
title_fullStr |
Polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitis |
title_full_unstemmed |
Polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitis |
title_sort |
polymorphisms in the interleukin-10 gene and relation to phenotype in patients with ulcerative colitis |
description |
Background and objectives: interleukin-10 (IL-10) has a key role in regulating mucosal inflammation in inflammatory bowel disease. In our population of Spanish ulcerative colitis (UC) patients, we have previously demostrated that two polymorphisms (IL-10.G14 microsatellite allele and homozygous for the -1082G alelle (guanine at position -1082)) in the IL-10 gene were susceptibility markers for disease. No data exist regarding the relationship of these IL-10 polymorphims with phenotypic subpopulations in UC. Therefore, this study sought to examine the contribution of IL-10 polymorphims to phenotypical variability in UC. Material and methods: a cohort of 215 Spanish unrelated patients with UC recruited in a single center was studied. All patients were rigorously phenotyped and followed for at least 3 years (mean time: 11.8 years). The clinical phenotype was established before genotyping. Genotyping was performed using polymerase chain reaction (PCR) assays. Results: patiens with UC included 129 (60%) men and 89 (40%) women. Mean age at diagnosis was 38 years, with a range of 8-83. Disease extent included 127 (59.1%) left-side patients and 88 (40.9%) extensive patients. Neither UC phenotype variable was associated with the presence of susceptibility polymorphims (10G14 microsatellite and -1082G alelle). Conclusions: in Madrid's Spanish population of UC patients, the carrying of the ILG14 microsatellite or -1082G polymorphism in the IL-10 gene was not associated with phenotype of disease. |
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Sociedad Española de Patología Digestiva |
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2006 |
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http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082006000200004 |
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