Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage

ABSTRACT Salinity is a serious problem that limits crop growth and yield. The present study used plotting to evaluate 25 tomato (Solanum lycopersicum L.) accessions for various morpho-physiological parameters at the seedling stage and identify significantly salt-tolerant tomato lines at three different salinity levels. The pot experiment had a completely randomized design with three replicates in a factorial arrangement under greenhouse conditions. Tomato plants were exposed to 0 (control), 8, and 12 dS m-1 as NaCl stress at the seedling stage. The morpho-physiological traits, such as root and shoot length, root/shoot ratio, number of leaves, fresh and dry shoot weight, fresh and dry root weight, leaf area, Na+ and K+ concentrations, K+/Na+ ratio, and tolerance index, were recorded to examine salt tolerance. According to principal component analysis (PCA), there were six principal components (PCs) with Eigen values > 1 and 77.2% of total cumulative variability. The PC1 (24.3%) revealed the highest variability followed by PC2 (16.2%). Meanwhile, the PCA biplot and cluster heat map analyses indicated that Subarctic, Raad-Red, Naqeeb, Pakit, Tommy-Toe, and BL-1076 were salt-tolerant, whereas PBLA-1401, PB-017902, CLN-2413, BL-1078, BL-1174, and BL-1079 were the most susceptible accessions based on their performance under stress.

Bibliographic Details

Main Authors:	Rehman,Fazal, Saeed,Asif, Yaseen,Muhammad, Shakeel,Amir, Ziaf,Khurram, Munir,Hassan, Tariq,Sultan Ali, Raza,Muhammad Ahsan, Riaz,Awais
Format:	Digital revista
Language:	English
Published:	Instituto de Investigaciones Agropecuarias, INIA 2019
Online Access:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100056
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