Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage
ABSTRACT Salinity is a serious problem that limits crop growth and yield. The present study used plotting to evaluate 25 tomato (Solanum lycopersicum L.) accessions for various morpho-physiological parameters at the seedling stage and identify significantly salt-tolerant tomato lines at three different salinity levels. The pot experiment had a completely randomized design with three replicates in a factorial arrangement under greenhouse conditions. Tomato plants were exposed to 0 (control), 8, and 12 dS m-1 as NaCl stress at the seedling stage. The morpho-physiological traits, such as root and shoot length, root/shoot ratio, number of leaves, fresh and dry shoot weight, fresh and dry root weight, leaf area, Na+ and K+ concentrations, K+/Na+ ratio, and tolerance index, were recorded to examine salt tolerance. According to principal component analysis (PCA), there were six principal components (PCs) with Eigen values > 1 and 77.2% of total cumulative variability. The PC1 (24.3%) revealed the highest variability followed by PC2 (16.2%). Meanwhile, the PCA biplot and cluster heat map analyses indicated that Subarctic, Raad-Red, Naqeeb, Pakit, Tommy-Toe, and BL-1076 were salt-tolerant, whereas PBLA-1401, PB-017902, CLN-2413, BL-1078, BL-1174, and BL-1079 were the most susceptible accessions based on their performance under stress.
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Instituto de Investigaciones Agropecuarias, INIA
2019
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oai:scielo:S0718-583920190001000562019-02-19Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stageRehman,FazalSaeed,AsifYaseen,MuhammadShakeel,AmirZiaf,KhurramMunir,HassanTariq,Sultan AliRaza,Muhammad AhsanRiaz,Awais Cluster heat map pot experiment principal component analysis salinity Solanum lycopersicum tomato seedling ABSTRACT Salinity is a serious problem that limits crop growth and yield. The present study used plotting to evaluate 25 tomato (Solanum lycopersicum L.) accessions for various morpho-physiological parameters at the seedling stage and identify significantly salt-tolerant tomato lines at three different salinity levels. The pot experiment had a completely randomized design with three replicates in a factorial arrangement under greenhouse conditions. Tomato plants were exposed to 0 (control), 8, and 12 dS m-1 as NaCl stress at the seedling stage. The morpho-physiological traits, such as root and shoot length, root/shoot ratio, number of leaves, fresh and dry shoot weight, fresh and dry root weight, leaf area, Na+ and K+ concentrations, K+/Na+ ratio, and tolerance index, were recorded to examine salt tolerance. According to principal component analysis (PCA), there were six principal components (PCs) with Eigen values > 1 and 77.2% of total cumulative variability. The PC1 (24.3%) revealed the highest variability followed by PC2 (16.2%). Meanwhile, the PCA biplot and cluster heat map analyses indicated that Subarctic, Raad-Red, Naqeeb, Pakit, Tommy-Toe, and BL-1076 were salt-tolerant, whereas PBLA-1401, PB-017902, CLN-2413, BL-1078, BL-1174, and BL-1079 were the most susceptible accessions based on their performance under stress.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.79 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100056en10.4067/S0718-58392019000100056 |
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Rehman,Fazal Saeed,Asif Yaseen,Muhammad Shakeel,Amir Ziaf,Khurram Munir,Hassan Tariq,Sultan Ali Raza,Muhammad Ahsan Riaz,Awais |
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Rehman,Fazal Saeed,Asif Yaseen,Muhammad Shakeel,Amir Ziaf,Khurram Munir,Hassan Tariq,Sultan Ali Raza,Muhammad Ahsan Riaz,Awais Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage |
author_facet |
Rehman,Fazal Saeed,Asif Yaseen,Muhammad Shakeel,Amir Ziaf,Khurram Munir,Hassan Tariq,Sultan Ali Raza,Muhammad Ahsan Riaz,Awais |
author_sort |
Rehman,Fazal |
title |
Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage |
title_short |
Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage |
title_full |
Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage |
title_fullStr |
Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage |
title_full_unstemmed |
Genetic evaluation and characterization using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage |
title_sort |
genetic evaluation and characterization using cluster heat map to assess nacl tolerance in tomato germplasm at the seedling stage |
description |
ABSTRACT Salinity is a serious problem that limits crop growth and yield. The present study used plotting to evaluate 25 tomato (Solanum lycopersicum L.) accessions for various morpho-physiological parameters at the seedling stage and identify significantly salt-tolerant tomato lines at three different salinity levels. The pot experiment had a completely randomized design with three replicates in a factorial arrangement under greenhouse conditions. Tomato plants were exposed to 0 (control), 8, and 12 dS m-1 as NaCl stress at the seedling stage. The morpho-physiological traits, such as root and shoot length, root/shoot ratio, number of leaves, fresh and dry shoot weight, fresh and dry root weight, leaf area, Na+ and K+ concentrations, K+/Na+ ratio, and tolerance index, were recorded to examine salt tolerance. According to principal component analysis (PCA), there were six principal components (PCs) with Eigen values > 1 and 77.2% of total cumulative variability. The PC1 (24.3%) revealed the highest variability followed by PC2 (16.2%). Meanwhile, the PCA biplot and cluster heat map analyses indicated that Subarctic, Raad-Red, Naqeeb, Pakit, Tommy-Toe, and BL-1076 were salt-tolerant, whereas PBLA-1401, PB-017902, CLN-2413, BL-1078, BL-1174, and BL-1079 were the most susceptible accessions based on their performance under stress. |
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Instituto de Investigaciones Agropecuarias, INIA |
publishDate |
2019 |
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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100056 |
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