Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion

The objective of this study was to determine the effects of coating nanoparticles of titanium dioxide (TiO2 NPs) and irradiation -UV on plates of titanium (Ti) for the adhesion and proliferation of human gingival fibroblasts (HGF). A total of 15 Ti plates were divided into three groups (n = 5); (i) control Ti, (ii) experimental: Ti+TiO2 NPs, (iii) experimental: Ti+TiO2 NPs+UV. The plates were analyzed with atomic force microscopy (AFM) and the roughness (Ra and Rmax) was determined. UV irradiation was performed for 20 min. HGF were subcultured in DMEM+10 % fetal bovine serum (FBS) at 37 °C with 5 % CO2. 2x106 cells/mL were inoculated on the plates and incubated for 1 h and washed with phosphate buffer saline (PBS). In the case of cell proliferation, cells were incubated for further 24 h more. Cell viability was determined with the MTT method, the formazan was dissolved with dimethylsulfoxide (DMSO) and analyzed at 540 nm. Experiments were performed of three independent experiments and data were analyzed by Kruskall-Wallis and multiple comparison of Mann-Whitney test. The surface topography of samples corresponded as follow: Ti (Ra= 0,492 µm y Rms= 0.640 µm), Ti+NPs TiO2, (Ra= 0.55 µm y Rms= 0.714 µm), respectively. The coating with TiO2 NPs significantly (p <0.05) increased the adhesion and proliferation of HGF compared with the group. The modification of Ti plates by coated with TiO2 NPs significantly increased adhesion and proliferation of HGF with the formation of a hydrophilic surface which favors the humectancy. This treatment may be reported here convenient to accelerate osseointegration of dental implants based titanium.

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Main Authors: Ornelas-Ponce,Adriana, Amezcua-Romero,Julio, Acosta-Torres,Laura S, Garcia-Contreras,Rene
Format: Digital revista
Language:English
Published: Universidad de La Frontera. Facultad de Medicina 2016
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2016000200008
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spelling oai:scielo:S0718-381X20160002000082016-09-23Effects of TiO2 NPs Coating-Titanium for Fibroblast AdhesionOrnelas-Ponce,AdrianaAmezcua-Romero,JulioAcosta-Torres,Laura SGarcia-Contreras,Rene titanium TiO2 nanoparticles UV irradiation cell adhesion human gingival fibroblast The objective of this study was to determine the effects of coating nanoparticles of titanium dioxide (TiO2 NPs) and irradiation -UV on plates of titanium (Ti) for the adhesion and proliferation of human gingival fibroblasts (HGF). A total of 15 Ti plates were divided into three groups (n = 5); (i) control Ti, (ii) experimental: Ti+TiO2 NPs, (iii) experimental: Ti+TiO2 NPs+UV. The plates were analyzed with atomic force microscopy (AFM) and the roughness (Ra and Rmax) was determined. UV irradiation was performed for 20 min. HGF were subcultured in DMEM+10 % fetal bovine serum (FBS) at 37 °C with 5 % CO2. 2x106 cells/mL were inoculated on the plates and incubated for 1 h and washed with phosphate buffer saline (PBS). In the case of cell proliferation, cells were incubated for further 24 h more. Cell viability was determined with the MTT method, the formazan was dissolved with dimethylsulfoxide (DMSO) and analyzed at 540 nm. Experiments were performed of three independent experiments and data were analyzed by Kruskall-Wallis and multiple comparison of Mann-Whitney test. The surface topography of samples corresponded as follow: Ti (Ra= 0,492 µm y Rms= 0.640 µm), Ti+NPs TiO2, (Ra= 0.55 µm y Rms= 0.714 µm), respectively. The coating with TiO2 NPs significantly (p <0.05) increased the adhesion and proliferation of HGF compared with the group. The modification of Ti plates by coated with TiO2 NPs significantly increased adhesion and proliferation of HGF with the formation of a hydrophilic surface which favors the humectancy. This treatment may be reported here convenient to accelerate osseointegration of dental implants based titanium.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.10 n.2 20162016-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2016000200008en10.4067/S0718-381X2016000200008
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country Chile
countrycode CL
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databasecode rev-scielo-cl
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region America del Sur
libraryname SciELO
language English
format Digital
author Ornelas-Ponce,Adriana
Amezcua-Romero,Julio
Acosta-Torres,Laura S
Garcia-Contreras,Rene
spellingShingle Ornelas-Ponce,Adriana
Amezcua-Romero,Julio
Acosta-Torres,Laura S
Garcia-Contreras,Rene
Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion
author_facet Ornelas-Ponce,Adriana
Amezcua-Romero,Julio
Acosta-Torres,Laura S
Garcia-Contreras,Rene
author_sort Ornelas-Ponce,Adriana
title Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion
title_short Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion
title_full Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion
title_fullStr Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion
title_full_unstemmed Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion
title_sort effects of tio2 nps coating-titanium for fibroblast adhesion
description The objective of this study was to determine the effects of coating nanoparticles of titanium dioxide (TiO2 NPs) and irradiation -UV on plates of titanium (Ti) for the adhesion and proliferation of human gingival fibroblasts (HGF). A total of 15 Ti plates were divided into three groups (n = 5); (i) control Ti, (ii) experimental: Ti+TiO2 NPs, (iii) experimental: Ti+TiO2 NPs+UV. The plates were analyzed with atomic force microscopy (AFM) and the roughness (Ra and Rmax) was determined. UV irradiation was performed for 20 min. HGF were subcultured in DMEM+10 % fetal bovine serum (FBS) at 37 °C with 5 % CO2. 2x106 cells/mL were inoculated on the plates and incubated for 1 h and washed with phosphate buffer saline (PBS). In the case of cell proliferation, cells were incubated for further 24 h more. Cell viability was determined with the MTT method, the formazan was dissolved with dimethylsulfoxide (DMSO) and analyzed at 540 nm. Experiments were performed of three independent experiments and data were analyzed by Kruskall-Wallis and multiple comparison of Mann-Whitney test. The surface topography of samples corresponded as follow: Ti (Ra= 0,492 µm y Rms= 0.640 µm), Ti+NPs TiO2, (Ra= 0.55 µm y Rms= 0.714 µm), respectively. The coating with TiO2 NPs significantly (p <0.05) increased the adhesion and proliferation of HGF compared with the group. The modification of Ti plates by coated with TiO2 NPs significantly increased adhesion and proliferation of HGF with the formation of a hydrophilic surface which favors the humectancy. This treatment may be reported here convenient to accelerate osseointegration of dental implants based titanium.
publisher Universidad de La Frontera. Facultad de Medicina
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2016000200008
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