METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal standard, were purified using a liquid - liquid extraction technique. The drugs were separated through a Kromasil 100-5C18 column with a mobile phase composed of acetonitrile and 0.02 M potassium dihydrogen phosphate (34 : 66 v/v) adjusted to pH 3.0 and quantified by fluorescence detection (excitation at 268 nm, emission at 410 nm). The calibration curve was linear ranging between 0.005 and 1.500 mg/mL. The detection limit was 0.001mg/mL and the quantification limit 0.003 mg/mL in plasma. The furosemide recovery from 1mL of plasma was between 99.8 and 101.6 %, with a variation coefficient not higher than 4,85 %. The method is sensitive enough for use in bioavailability and bioequivalence studies after oral administration of 40 mg (a tablet) of furosemide
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Sociedad Chilena de Química
2005
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oai:scielo:S0717-970720050002000082005-07-19METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTIONGOMEZ G,CAROLINAPLESSING R,CARLOS vonGODOY M,C. GLORIAREINBACH H,ROLANDOGODOY R,RICARDO 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid urosemide liquid-liquid extraction from plasma HPLC with fluorescence detection validation A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal standard, were purified using a liquid - liquid extraction technique. The drugs were separated through a Kromasil 100-5C18 column with a mobile phase composed of acetonitrile and 0.02 M potassium dihydrogen phosphate (34 : 66 v/v) adjusted to pH 3.0 and quantified by fluorescence detection (excitation at 268 nm, emission at 410 nm). The calibration curve was linear ranging between 0.005 and 1.500 mg/mL. The detection limit was 0.001mg/mL and the quantification limit 0.003 mg/mL in plasma. The furosemide recovery from 1mL of plasma was between 99.8 and 101.6 %, with a variation coefficient not higher than 4,85 %. The method is sensitive enough for use in bioavailability and bioequivalence studies after oral administration of 40 mg (a tablet) of furosemideinfo:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.50 n.2 20052005-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200008en10.4067/S0717-97072005000200008 |
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GOMEZ G,CAROLINA PLESSING R,CARLOS von GODOY M,C. GLORIA REINBACH H,ROLANDO GODOY R,RICARDO |
spellingShingle |
GOMEZ G,CAROLINA PLESSING R,CARLOS von GODOY M,C. GLORIA REINBACH H,ROLANDO GODOY R,RICARDO METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION |
author_facet |
GOMEZ G,CAROLINA PLESSING R,CARLOS von GODOY M,C. GLORIA REINBACH H,ROLANDO GODOY R,RICARDO |
author_sort |
GOMEZ G,CAROLINA |
title |
METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION |
title_short |
METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION |
title_full |
METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION |
title_fullStr |
METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION |
title_full_unstemmed |
METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION |
title_sort |
method validation for the determination of furosemide in plasma by liquid- liquid extraction and high-performance liquid chromatography with fluorescence detection |
description |
A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal standard, were purified using a liquid - liquid extraction technique. The drugs were separated through a Kromasil 100-5C18 column with a mobile phase composed of acetonitrile and 0.02 M potassium dihydrogen phosphate (34 : 66 v/v) adjusted to pH 3.0 and quantified by fluorescence detection (excitation at 268 nm, emission at 410 nm). The calibration curve was linear ranging between 0.005 and 1.500 mg/mL. The detection limit was 0.001mg/mL and the quantification limit 0.003 mg/mL in plasma. The furosemide recovery from 1mL of plasma was between 99.8 and 101.6 %, with a variation coefficient not higher than 4,85 %. The method is sensitive enough for use in bioavailability and bioequivalence studies after oral administration of 40 mg (a tablet) of furosemide |
publisher |
Sociedad Chilena de Química |
publishDate |
2005 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072005000200008 |
work_keys_str_mv |
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