Optimization of β-carotene production by a newly isolated Serratia marcescens strain
β-carotene is a commonly used food colorant. In this work, a novel β-carotene producing strain, Serratia marcescens RB3, was isolated and identified by physiological and biochemical tests, as well as 16S rDNA sequence analysis. The production of β-carotene by S. marcescens RB3 was identified through HPLC analysis. The cultivation conditions for β-carotene production by S. marcescens RB3 were optimized as 2.0% lactose, 2.0% peptone, 0.3% beef extract, 1.0% NaCl supplemented with 0.05% Fe2+, pH 6.0 and 30ºC. Under the optimal conditions, the yield of β-carotene achieved 2.45 µg/mL.
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Pontificia Universidad Católica de Valparaíso
2012
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oai:scielo:S0717-345820120006000032013-01-14Optimization of β-carotene production by a newly isolated Serratia marcescens strainWang,BohuaLin,LipingLu,LeiChen,Weiping β-carotene optimization pigment Serratia marcescens β-carotene is a commonly used food colorant. In this work, a novel β-carotene producing strain, Serratia marcescens RB3, was isolated and identified by physiological and biochemical tests, as well as 16S rDNA sequence analysis. The production of β-carotene by S. marcescens RB3 was identified through HPLC analysis. The cultivation conditions for β-carotene production by S. marcescens RB3 were optimized as 2.0% lactose, 2.0% peptone, 0.3% beef extract, 1.0% NaCl supplemented with 0.05% Fe2+, pH 6.0 and 30ºC. Under the optimal conditions, the yield of β-carotene achieved 2.45 µg/mL.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.6 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600003en10.2225/vol15-issue6-fulltext-4 |
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Wang,Bohua Lin,Liping Lu,Lei Chen,Weiping |
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Wang,Bohua Lin,Liping Lu,Lei Chen,Weiping Optimization of β-carotene production by a newly isolated Serratia marcescens strain |
| author_facet |
Wang,Bohua Lin,Liping Lu,Lei Chen,Weiping |
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Wang,Bohua |
| title |
Optimization of β-carotene production by a newly isolated Serratia marcescens strain |
| title_short |
Optimization of β-carotene production by a newly isolated Serratia marcescens strain |
| title_full |
Optimization of β-carotene production by a newly isolated Serratia marcescens strain |
| title_fullStr |
Optimization of β-carotene production by a newly isolated Serratia marcescens strain |
| title_full_unstemmed |
Optimization of β-carotene production by a newly isolated Serratia marcescens strain |
| title_sort |
optimization of β-carotene production by a newly isolated serratia marcescens strain |
| description |
β-carotene is a commonly used food colorant. In this work, a novel β-carotene producing strain, Serratia marcescens RB3, was isolated and identified by physiological and biochemical tests, as well as 16S rDNA sequence analysis. The production of β-carotene by S. marcescens RB3 was identified through HPLC analysis. The cultivation conditions for β-carotene production by S. marcescens RB3 were optimized as 2.0% lactose, 2.0% peptone, 0.3% beef extract, 1.0% NaCl supplemented with 0.05% Fe2+, pH 6.0 and 30ºC. Under the optimal conditions, the yield of β-carotene achieved 2.45 µg/mL. |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2012 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600003 |
| work_keys_str_mv |
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| _version_ |
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