Production of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activities
Lignocellulolytic enzyme production by Aspergillus niger was compared both in submerged fermentation (SF) and biofilm fermentation (BF) at varying water activities. Maximal filter paper activity, endoglucanase and xylanase activities were much higher in BF (2.96, 4.7 and 4.61 IU ml-1, respectively) than in SF cultures (1.71, 1.31 and 2.3 IU ml-1, respectively) but biomass yields were lower in BF than in SF (0.338 g g-1 and 0.431 g g-1, respectively). In the presence of 20% ethylene glycol (a w = 0.942) the enzyme activities decreased in both systems but BF still had higher levels (1.0, 1.0 and 2.6 IU ml-1, respectively) than SF cultures (0.6, 0.7 and 1.5 IU ml-1, respectively). An increase in xylanase specific activity of more than 2 fold (from 4.2 to 10.2 IU mg-1 biomass) was observed in the presence of 20% ethylene glycol, suggesting differential regulatory mechanisms in biofilm fermentation related to cell adhesion.
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Pontificia Universidad Católica de Valparaíso
2007
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oai:scielo:S0717-345820070001000122007-08-14Production of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activitiesVillena,Gretty KGutiérrez-Correa,Marcel biofilm cellulase ethylene glycol water activity xylanase Lignocellulolytic enzyme production by Aspergillus niger was compared both in submerged fermentation (SF) and biofilm fermentation (BF) at varying water activities. Maximal filter paper activity, endoglucanase and xylanase activities were much higher in BF (2.96, 4.7 and 4.61 IU ml-1, respectively) than in SF cultures (1.71, 1.31 and 2.3 IU ml-1, respectively) but biomass yields were lower in BF than in SF (0.338 g g-1 and 0.431 g g-1, respectively). In the presence of 20% ethylene glycol (a w = 0.942) the enzyme activities decreased in both systems but BF still had higher levels (1.0, 1.0 and 2.6 IU ml-1, respectively) than SF cultures (0.6, 0.7 and 1.5 IU ml-1, respectively). An increase in xylanase specific activity of more than 2 fold (from 4.2 to 10.2 IU mg-1 biomass) was observed in the presence of 20% ethylene glycol, suggesting differential regulatory mechanisms in biofilm fermentation related to cell adhesion.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100012en10.4067/S0717-34582007000100012 |
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Villena,Gretty K Gutiérrez-Correa,Marcel |
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Villena,Gretty K Gutiérrez-Correa,Marcel Production of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activities |
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Villena,Gretty K Gutiérrez-Correa,Marcel |
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Villena,Gretty K |
title |
Production of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activities |
title_short |
Production of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activities |
title_full |
Production of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activities |
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Production of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activities |
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Production of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activities |
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production of lignocellulolytic enzymes by aspergillus niger biofilms at variable water activities |
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Lignocellulolytic enzyme production by Aspergillus niger was compared both in submerged fermentation (SF) and biofilm fermentation (BF) at varying water activities. Maximal filter paper activity, endoglucanase and xylanase activities were much higher in BF (2.96, 4.7 and 4.61 IU ml-1, respectively) than in SF cultures (1.71, 1.31 and 2.3 IU ml-1, respectively) but biomass yields were lower in BF than in SF (0.338 g g-1 and 0.431 g g-1, respectively). In the presence of 20% ethylene glycol (a w = 0.942) the enzyme activities decreased in both systems but BF still had higher levels (1.0, 1.0 and 2.6 IU ml-1, respectively) than SF cultures (0.6, 0.7 and 1.5 IU ml-1, respectively). An increase in xylanase specific activity of more than 2 fold (from 4.2 to 10.2 IU mg-1 biomass) was observed in the presence of 20% ethylene glycol, suggesting differential regulatory mechanisms in biofilm fermentation related to cell adhesion. |
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Pontificia Universidad Católica de Valparaíso |
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2007 |
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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100012 |
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AT villenagrettyk productionoflignocellulolyticenzymesbyaspergillusnigerbiofilmsatvariablewateractivities AT gutierrezcorreamarcel productionoflignocellulolyticenzymesbyaspergillusnigerbiofilmsatvariablewateractivities |
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