Catalase enzyme in mitochondria of Saccharomyces cerevisiae
Catalase and superoxide dismutase activities have been explored in the yeast Saccharomyces cerevisiae during batchwise growth experiment. During the diauxic growth in YPD medium high Ys values were obtained (0.415 - 0.423) and correlation between the total activities of both enzymes has been found. A mitochondrial fraction from three type strains of Saccharomyces cerevisiae has been isolated. The purity of this fraction was proved through different enzyme assays: hexokinase, glucose-6-phosphate dehydrogenase, D-amino acid oxidase, isocitric lyase, succinate dehydrogenase. Then the catalase, peroxidase, Mn and Cu/Zn superoxide dismutase activities were evaluated in the mitochondrial fraction. Polyacrylamide gel electrophoresis separations allowed to identify a mitochondrial catalase as a band of 0.239 Rm value. It differed from the two catalase specific bands with Rm values 0.218 and 0.257 obtained from the crude extract. It was proved that the three catalase proteins are charge isomers. A positive correlation between the activity of mitochondrial catalase and Mn superoxide dismutase also takes place. Molecular weight of mitochonrial catalase protein has been determined as 240 kD.
Main Authors: | Petrova,Ventsislava Yankova, Rasheva,Tanya Vassileva, Kujumdzieva,Anna V. |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Pontificia Universidad Católica de Valparaíso
2002
|
Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100010 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Nitrogenase cofactor biosynthesis using proteins produced in mitochondria of Saccharomyces cerevisiae
by: Dobrzyńska, Katarzyna, et al.
Published: (2024-02-14) -
High levels of catalase in sod mutants of Saccharomyces cerevisiae in high aeration conditions
by: Martins,Vanessa, et al.
Published: (2005) -
Enzyme complex and Saccharomyces cerevisiae in diets for broilers in the initial phase
by: Sousa,Regina Fialho de, et al.
Published: (2018) -
Filamentous growth in Saccharomyces cerevisiae
by: Ceccato-Antonini,Sandra Regina, et al.
Published: (2004) -
The correlation between the central carbon metabolic flux distribution and the number of shared enzyme regulators in Saccharomyces cerevisiae
by: Zhou,Xiangfei, et al.
Published: (2016)