Characterization of mitochondrial iron uptake in HepG2 cells
There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content.
Main Authors: | MAZARIEGOS,DORA INÉS, CABANTCHIK,Z. IOAV, CASSELS,BRUCE K, NÚÑEZ,MARCO TULIO |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedad de Biología de Chile
2006
|
Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Isocryptolepine, an indoloquinoline alkaloid from Cryptolepis sanguinolenta promotes LDL uptake in HepG2 cells
by: Aggrey,Mike Okweesi, et al.
Published: (2018) -
Uptake, Metabolism and Biological Effect of the Olive Oil Phenol Hydroxytyrosol by Human HepG2 Cells
by: Goya, Luis, et al.
Published: (2010) -
Time-course regulation of survival pathways by epicatechin on HepG2 cells
by: Granado-Serrano, Ana B., et al.
Published: (2009) -
Essential oil from Euphorbia esula inhibits proliferation and induces apoptosis in HepG2 cells via mitochondrial dysfunction
by: Lv,Dan, et al.
Published: (2020) -
Anti-proliferative effects of paroxetine alone or in combination with sorafenib in HepG2 cells
by: Cakil,Yaprak Donmez, et al.
Published: (2022)