Calidad microbiológica de una fórmula enteral lista para usar

Objective: To determinate and compare the microbiological quality of a ready to use enteral formula (EF): liquid ADN&trade; during different periods of time. Methods: The study was developed in the Hospital formula-preparing room. Twenty liters of EF were delivered in 40 plastic sterile bottles using aseptic technique, and were maintained at room temperature during 24 hours. Feed samples of 50 ml at time 0 and at 24 hours were obtained and frozen at _70º C, until they were investigated (40 feed samples of EF were cultivated at preparation time 0, and 24 hours). Mesophile count (Me), total coliform (TC) and faecal coliform (FC) bacteria were investigated. The feed samples were analized at the Microbiologic Laboratory of CESMEC. The microbial quality standards (MQS) were at time 0: < 10² UFC/ml of (Me), and no (TC) and (FC). At 24 hours: < 10³ UFC/ml of (Me), < 10 UFC/ml of (TC) and no (FC).The statistical data analysis was done using Stata program and Z test was used for proportions. The level of p < 0,05 was considered statistically significant. Results: The average of fulfilment (MQS) for liquid ADN&trade; at time 0, and 24 hours was 100% and 95% (p = 0,3) for Me. TC got 100% fulfilment (MQS) at any time. FC were not detected at any time. Conclusions: Liquid ADN&trade;can be hung during 24 hours at room temperature.

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Main Authors: Kehr S.,Juan, Morales V.,Blanca, Contreras Sch.,Paula, Castillo D.,Loriana, Aranda Ch.,Waldo
Format: Digital revista
Language:Spanish / Castilian
Published: Sociedad Chilena de Infectología 2004
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182004000400005
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spelling oai:scielo:S0716-101820040004000052005-04-14Calidad microbiológica de una fórmula enteral lista para usarKehr S.,JuanMorales V.,BlancaContreras Sch.,PaulaCastillo D.,LorianaAranda Ch.,Waldo contaminación bacteriana alimentación enteral ADN líquido® Objective: To determinate and compare the microbiological quality of a ready to use enteral formula (EF): liquid ADN&trade; during different periods of time. Methods: The study was developed in the Hospital formula-preparing room. Twenty liters of EF were delivered in 40 plastic sterile bottles using aseptic technique, and were maintained at room temperature during 24 hours. Feed samples of 50 ml at time 0 and at 24 hours were obtained and frozen at _70º C, until they were investigated (40 feed samples of EF were cultivated at preparation time 0, and 24 hours). Mesophile count (Me), total coliform (TC) and faecal coliform (FC) bacteria were investigated. The feed samples were analized at the Microbiologic Laboratory of CESMEC. The microbial quality standards (MQS) were at time 0: < 10² UFC/ml of (Me), and no (TC) and (FC). At 24 hours: < 10³ UFC/ml of (Me), < 10 UFC/ml of (TC) and no (FC).The statistical data analysis was done using Stata program and Z test was used for proportions. The level of p < 0,05 was considered statistically significant. Results: The average of fulfilment (MQS) for liquid ADN&trade; at time 0, and 24 hours was 100% and 95% (p = 0,3) for Me. TC got 100% fulfilment (MQS) at any time. FC were not detected at any time. Conclusions: Liquid ADN&trade;can be hung during 24 hours at room temperature.info:eu-repo/semantics/openAccessSociedad Chilena de InfectologíaRevista chilena de infectología v.21 n.4 20042004-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182004000400005es10.4067/S0716-10182004000400005
institution SCIELO
collection OJS
country Chile
countrycode CL
component Revista
access En linea
databasecode rev-scielo-cl
tag revista
region America del Sur
libraryname SciELO
language Spanish / Castilian
format Digital
author Kehr S.,Juan
Morales V.,Blanca
Contreras Sch.,Paula
Castillo D.,Loriana
Aranda Ch.,Waldo
spellingShingle Kehr S.,Juan
Morales V.,Blanca
Contreras Sch.,Paula
Castillo D.,Loriana
Aranda Ch.,Waldo
Calidad microbiológica de una fórmula enteral lista para usar
author_facet Kehr S.,Juan
Morales V.,Blanca
Contreras Sch.,Paula
Castillo D.,Loriana
Aranda Ch.,Waldo
author_sort Kehr S.,Juan
title Calidad microbiológica de una fórmula enteral lista para usar
title_short Calidad microbiológica de una fórmula enteral lista para usar
title_full Calidad microbiológica de una fórmula enteral lista para usar
title_fullStr Calidad microbiológica de una fórmula enteral lista para usar
title_full_unstemmed Calidad microbiológica de una fórmula enteral lista para usar
title_sort calidad microbiológica de una fórmula enteral lista para usar
description Objective: To determinate and compare the microbiological quality of a ready to use enteral formula (EF): liquid ADN&trade; during different periods of time. Methods: The study was developed in the Hospital formula-preparing room. Twenty liters of EF were delivered in 40 plastic sterile bottles using aseptic technique, and were maintained at room temperature during 24 hours. Feed samples of 50 ml at time 0 and at 24 hours were obtained and frozen at _70º C, until they were investigated (40 feed samples of EF were cultivated at preparation time 0, and 24 hours). Mesophile count (Me), total coliform (TC) and faecal coliform (FC) bacteria were investigated. The feed samples were analized at the Microbiologic Laboratory of CESMEC. The microbial quality standards (MQS) were at time 0: < 10² UFC/ml of (Me), and no (TC) and (FC). At 24 hours: < 10³ UFC/ml of (Me), < 10 UFC/ml of (TC) and no (FC).The statistical data analysis was done using Stata program and Z test was used for proportions. The level of p < 0,05 was considered statistically significant. Results: The average of fulfilment (MQS) for liquid ADN&trade; at time 0, and 24 hours was 100% and 95% (p = 0,3) for Me. TC got 100% fulfilment (MQS) at any time. FC were not detected at any time. Conclusions: Liquid ADN&trade;can be hung during 24 hours at room temperature.
publisher Sociedad Chilena de Infectología
publishDate 2004
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182004000400005
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