Immunophenotype characteristics of peripheral blood mononuclear leukocytes of Chronic Idiopathic Urticaria patients

The pathogenesis of chronic idiopathic urticaria (CIU) is not completely understood although autoimmunity has been proposed. The aim of the study was to assess the expression of different leukocyte antigens, by flow cytometry, assaying total blood of 29 patients with CIU and of 20 sex and age matched controls. Moreover, we assessed soluble CD154 a marker of immune cell activation, predominantly memory T cells. When patients were divided depending an their response to the autologous serum skin test (ASST), three different groups were encountered: group 1 (n = 11): with negative ASST -, group 2 (n = 11): positive ASST (ASST +) with normal lymphocyte counts and group 3 (n = 7): ASST + with low lymphocyte counts (< 1500 cells/mm³). A significant increase in CD19+ percentage and not in the absolute count (P < 0.05) was observed in group 1 as compared to controls and to the other groups. In contrast, CD30+, CD45RO+ and CD4+/CD45RO+ percentages and biologically active soluble CD154 levels were significantly higher (P < 0.05) in group 3 as compared to group 1 or to controls. In ASST positive groups, CD45RO+ and CD4+/CD45RO+ positiveness correlates with wheal diameter. In conclusion, memory cells may play a role in these different types of patients and in understanding CIU pathogenesis.

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Main Authors: Garmendia,Jenny V, Zabaleta,Mercedes, Aldrey,Oscar, Rivera,Henry, De Sanctis,Juan B, Bianco,Nicolás E, Blanca,Isaac
Format: Digital revista
Language:English
Published: Instituto de Investigaciones Clínicas "Dr. Américo Negrette", Facultad de Medicina, Universidad del Zulia 2006
Online Access:http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0535-51332006000400005
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spelling oai:scielo:S0535-513320060004000052007-01-22Immunophenotype characteristics of peripheral blood mononuclear leukocytes of Chronic Idiopathic Urticaria patientsGarmendia,Jenny VZabaleta,MercedesAldrey,OscarRivera,HenryDe Sanctis,Juan BBianco,Nicolás EBlanca,Isaac Urticaria cell immunophenotype memory cells The pathogenesis of chronic idiopathic urticaria (CIU) is not completely understood although autoimmunity has been proposed. The aim of the study was to assess the expression of different leukocyte antigens, by flow cytometry, assaying total blood of 29 patients with CIU and of 20 sex and age matched controls. Moreover, we assessed soluble CD154 a marker of immune cell activation, predominantly memory T cells. When patients were divided depending an their response to the autologous serum skin test (ASST), three different groups were encountered: group 1 (n = 11): with negative ASST -, group 2 (n = 11): positive ASST (ASST +) with normal lymphocyte counts and group 3 (n = 7): ASST + with low lymphocyte counts (< 1500 cells/mm³). A significant increase in CD19+ percentage and not in the absolute count (P < 0.05) was observed in group 1 as compared to controls and to the other groups. In contrast, CD30+, CD45RO+ and CD4+/CD45RO+ percentages and biologically active soluble CD154 levels were significantly higher (P < 0.05) in group 3 as compared to group 1 or to controls. In ASST positive groups, CD45RO+ and CD4+/CD45RO+ positiveness correlates with wheal diameter. In conclusion, memory cells may play a role in these different types of patients and in understanding CIU pathogenesis.info:eu-repo/semantics/openAccessInstituto de Investigaciones Clínicas "Dr. Américo Negrette", Facultad de Medicina, Universidad del ZuliaInvestigación Clínica v.47 n.4 20062006-12-01info:eu-repo/semantics/articletext/htmlhttp://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0535-51332006000400005en
institution SCIELO
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country Venezuela
countrycode VE
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databasecode rev-scielo-ve
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region America del Sur
libraryname SciELO
language English
format Digital
author Garmendia,Jenny V
Zabaleta,Mercedes
Aldrey,Oscar
Rivera,Henry
De Sanctis,Juan B
Bianco,Nicolás E
Blanca,Isaac
spellingShingle Garmendia,Jenny V
Zabaleta,Mercedes
Aldrey,Oscar
Rivera,Henry
De Sanctis,Juan B
Bianco,Nicolás E
Blanca,Isaac
Immunophenotype characteristics of peripheral blood mononuclear leukocytes of Chronic Idiopathic Urticaria patients
author_facet Garmendia,Jenny V
Zabaleta,Mercedes
Aldrey,Oscar
Rivera,Henry
De Sanctis,Juan B
Bianco,Nicolás E
Blanca,Isaac
author_sort Garmendia,Jenny V
title Immunophenotype characteristics of peripheral blood mononuclear leukocytes of Chronic Idiopathic Urticaria patients
title_short Immunophenotype characteristics of peripheral blood mononuclear leukocytes of Chronic Idiopathic Urticaria patients
title_full Immunophenotype characteristics of peripheral blood mononuclear leukocytes of Chronic Idiopathic Urticaria patients
title_fullStr Immunophenotype characteristics of peripheral blood mononuclear leukocytes of Chronic Idiopathic Urticaria patients
title_full_unstemmed Immunophenotype characteristics of peripheral blood mononuclear leukocytes of Chronic Idiopathic Urticaria patients
title_sort immunophenotype characteristics of peripheral blood mononuclear leukocytes of chronic idiopathic urticaria patients
description The pathogenesis of chronic idiopathic urticaria (CIU) is not completely understood although autoimmunity has been proposed. The aim of the study was to assess the expression of different leukocyte antigens, by flow cytometry, assaying total blood of 29 patients with CIU and of 20 sex and age matched controls. Moreover, we assessed soluble CD154 a marker of immune cell activation, predominantly memory T cells. When patients were divided depending an their response to the autologous serum skin test (ASST), three different groups were encountered: group 1 (n = 11): with negative ASST -, group 2 (n = 11): positive ASST (ASST +) with normal lymphocyte counts and group 3 (n = 7): ASST + with low lymphocyte counts (< 1500 cells/mm³). A significant increase in CD19+ percentage and not in the absolute count (P < 0.05) was observed in group 1 as compared to controls and to the other groups. In contrast, CD30+, CD45RO+ and CD4+/CD45RO+ percentages and biologically active soluble CD154 levels were significantly higher (P < 0.05) in group 3 as compared to group 1 or to controls. In ASST positive groups, CD45RO+ and CD4+/CD45RO+ positiveness correlates with wheal diameter. In conclusion, memory cells may play a role in these different types of patients and in understanding CIU pathogenesis.
publisher Instituto de Investigaciones Clínicas "Dr. Américo Negrette", Facultad de Medicina, Universidad del Zulia
publishDate 2006
url http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0535-51332006000400005
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