Neuroprotective effect of Sapucaia nuts (Lecythis pisonis) on rats fed with high-fat diet
Background: Lecythis pisonis Cambess is commonly known as "castanha de sapucaia" in Brazil. Chemical composition studies revealed that this nut is an excellent source of anti-oxidant minerals and of essential lipids. Objective: The aim of the present study is to assess the anti-oxidant and anti-inflammatory effect of Lecythis pisonis Cambess on the brain tissue of Wistar rats. Material and methods: The animals were divided in four experimental groups (n = 6), total of forty-eight rats. Treatments included the standard diet (AIN-93G) and high-fat food, supplemented with Sapucaia nut from 14 to 28 days. The gene expression markers TNF-α, NFkB, ZnSOD and HSP-72 were defined through reverse transcriptase polymerase chain reaction (rtPCR). The anti-oxidant effect was assessed through the thiobarbituric acid-reactive substances (TBARS) and the measurement of the activity performed by superoxide dismutase enzymes. Results: Accordingly, the gene expression of the inflammatory markers NFkB (p65) and TNF-α was lower in rats fed on diets supplemented with "sapucaia", and they presented significant difference in the Tukey test (p < 0.05). The heat-shock HSP-72 protein and the ZnSOD enzyme raised the gene expression and showed significant statistical difference (p < 0.05) in both groups fed on Sapucaia nut-based diet. Conclusion: Thus, the nutritional properties of the Sapucaia nuts perform important neuroprotective activities because they modulated the anti-oxidant activity and the brain tissue inflammatory process in the assessed animals.
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Grupo Arán
2016
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oai:scielo:S0212-161120160006000272017-03-28Neuroprotective effect of Sapucaia nuts (Lecythis pisonis) on rats fed with high-fat dietVidal Martins,MarcosMontezano de Carvalho,Izabela MariaMagalhães Caetano,Mônica MariaLopes Toledo,Renata CeliAvelar Xavier,AntônioQueiroz,José Humberto de Brain tissue Oxidative stress Inflammation Gene expression Background: Lecythis pisonis Cambess is commonly known as "castanha de sapucaia" in Brazil. Chemical composition studies revealed that this nut is an excellent source of anti-oxidant minerals and of essential lipids. Objective: The aim of the present study is to assess the anti-oxidant and anti-inflammatory effect of Lecythis pisonis Cambess on the brain tissue of Wistar rats. Material and methods: The animals were divided in four experimental groups (n = 6), total of forty-eight rats. Treatments included the standard diet (AIN-93G) and high-fat food, supplemented with Sapucaia nut from 14 to 28 days. The gene expression markers TNF-α, NFkB, ZnSOD and HSP-72 were defined through reverse transcriptase polymerase chain reaction (rtPCR). The anti-oxidant effect was assessed through the thiobarbituric acid-reactive substances (TBARS) and the measurement of the activity performed by superoxide dismutase enzymes. Results: Accordingly, the gene expression of the inflammatory markers NFkB (p65) and TNF-α was lower in rats fed on diets supplemented with "sapucaia", and they presented significant difference in the Tukey test (p < 0.05). The heat-shock HSP-72 protein and the ZnSOD enzyme raised the gene expression and showed significant statistical difference (p < 0.05) in both groups fed on Sapucaia nut-based diet. Conclusion: Thus, the nutritional properties of the Sapucaia nuts perform important neuroprotective activities because they modulated the anti-oxidant activity and the brain tissue inflammatory process in the assessed animals.Grupo AránNutrición Hospitalaria v.33 n.6 20162016-12-01journal articletext/htmlhttp://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S0212-16112016000600027en |
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Vidal Martins,Marcos Montezano de Carvalho,Izabela Maria Magalhães Caetano,Mônica Maria Lopes Toledo,Renata Celi Avelar Xavier,Antônio Queiroz,José Humberto de |
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Vidal Martins,Marcos Montezano de Carvalho,Izabela Maria Magalhães Caetano,Mônica Maria Lopes Toledo,Renata Celi Avelar Xavier,Antônio Queiroz,José Humberto de Neuroprotective effect of Sapucaia nuts (Lecythis pisonis) on rats fed with high-fat diet |
| author_facet |
Vidal Martins,Marcos Montezano de Carvalho,Izabela Maria Magalhães Caetano,Mônica Maria Lopes Toledo,Renata Celi Avelar Xavier,Antônio Queiroz,José Humberto de |
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Vidal Martins,Marcos |
| title |
Neuroprotective effect of Sapucaia nuts (Lecythis pisonis) on rats fed with high-fat diet |
| title_short |
Neuroprotective effect of Sapucaia nuts (Lecythis pisonis) on rats fed with high-fat diet |
| title_full |
Neuroprotective effect of Sapucaia nuts (Lecythis pisonis) on rats fed with high-fat diet |
| title_fullStr |
Neuroprotective effect of Sapucaia nuts (Lecythis pisonis) on rats fed with high-fat diet |
| title_full_unstemmed |
Neuroprotective effect of Sapucaia nuts (Lecythis pisonis) on rats fed with high-fat diet |
| title_sort |
neuroprotective effect of sapucaia nuts (lecythis pisonis) on rats fed with high-fat diet |
| description |
Background: Lecythis pisonis Cambess is commonly known as "castanha de sapucaia" in Brazil. Chemical composition studies revealed that this nut is an excellent source of anti-oxidant minerals and of essential lipids. Objective: The aim of the present study is to assess the anti-oxidant and anti-inflammatory effect of Lecythis pisonis Cambess on the brain tissue of Wistar rats. Material and methods: The animals were divided in four experimental groups (n = 6), total of forty-eight rats. Treatments included the standard diet (AIN-93G) and high-fat food, supplemented with Sapucaia nut from 14 to 28 days. The gene expression markers TNF-α, NFkB, ZnSOD and HSP-72 were defined through reverse transcriptase polymerase chain reaction (rtPCR). The anti-oxidant effect was assessed through the thiobarbituric acid-reactive substances (TBARS) and the measurement of the activity performed by superoxide dismutase enzymes. Results: Accordingly, the gene expression of the inflammatory markers NFkB (p65) and TNF-α was lower in rats fed on diets supplemented with "sapucaia", and they presented significant difference in the Tukey test (p < 0.05). The heat-shock HSP-72 protein and the ZnSOD enzyme raised the gene expression and showed significant statistical difference (p < 0.05) in both groups fed on Sapucaia nut-based diet. Conclusion: Thus, the nutritional properties of the Sapucaia nuts perform important neuroprotective activities because they modulated the anti-oxidant activity and the brain tissue inflammatory process in the assessed animals. |
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Grupo Arán |
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2016 |
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http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S0212-16112016000600027 |
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