PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE

ABSTRACT This study demonstrates that using beeswax hydrolyzates (Hw), short- or medium-chain-length polyhydroxyalkanoates (scl-mcl-PHA) can be obtained by a three stages fed-batch fermentation system. The fed batch fermentations were conducted with a strain of Cupriavidus necator. Glucose and ammonium sulphate as carbon and nitrogen sources, respectively, were used in a first and second culture stages. In the third stage (production of scl-mcl-PHA) a fresh medium was fed with Hw as a carbon source at a concentration of 5 g/L, obtaining 8.91 g/L of intracellular scl-mcl-PHA and 13.64 g/L of biomass, which resulted in 65.3 % w/w of scl-mcl-PHA. Using nuclear magnetic resonance, the presence of scl and mcl monomers, 3-hydroxybutyrate (3HB) and 3-hydroxydecanoate (3HD) was determined. The percentage of comonomer incorporation different to 3HB was 4.6 mol %. A glass transition temperature (Tg) of -50 ºC and a melting temperature (Tm) of 79.8 ºC were determined for the scl-mcl-PHA (3HB-co-3HDD).

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Main Authors: Quintanar-Gómez,Samuel, Abreu-Corona,Arturo, Zamudio-Pérez,Evelyn, Vargas-Hernández,Genaro, Téllez-Jurado,Alejandro, Gracida-Rodríguez,Jorge
Format: Digital revista
Language:English
Published: Universidad Nacional Autónoma de México, Centro de Ciencias de la Atmósfera 2018
Online Access:http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0188-49992018000300467
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spelling oai:scielo:S0188-499920180003004672019-03-27PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCEQuintanar-Gómez,SamuelAbreu-Corona,ArturoZamudio-Pérez,EvelynVargas-Hernández,GenaroTéllez-Jurado,AlejandroGracida-Rodríguez,Jorge biopolymers DSC mcl-PHA NMR fed-batch fermentation ABSTRACT This study demonstrates that using beeswax hydrolyzates (Hw), short- or medium-chain-length polyhydroxyalkanoates (scl-mcl-PHA) can be obtained by a three stages fed-batch fermentation system. The fed batch fermentations were conducted with a strain of Cupriavidus necator. Glucose and ammonium sulphate as carbon and nitrogen sources, respectively, were used in a first and second culture stages. In the third stage (production of scl-mcl-PHA) a fresh medium was fed with Hw as a carbon source at a concentration of 5 g/L, obtaining 8.91 g/L of intracellular scl-mcl-PHA and 13.64 g/L of biomass, which resulted in 65.3 % w/w of scl-mcl-PHA. Using nuclear magnetic resonance, the presence of scl and mcl monomers, 3-hydroxybutyrate (3HB) and 3-hydroxydecanoate (3HD) was determined. The percentage of comonomer incorporation different to 3HB was 4.6 mol %. A glass transition temperature (Tg) of -50 ºC and a melting temperature (Tm) of 79.8 ºC were determined for the scl-mcl-PHA (3HB-co-3HDD).info:eu-repo/semantics/openAccessUniversidad Nacional Autónoma de México, Centro de Ciencias de la AtmósferaRevista internacional de contaminación ambiental v.34 n.3 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0188-49992018000300467en10.20937/rica.2018.34.03.09
institution SCIELO
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country México
countrycode MX
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access En linea
databasecode rev-scielo-mx
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region America del Norte
libraryname SciELO
language English
format Digital
author Quintanar-Gómez,Samuel
Abreu-Corona,Arturo
Zamudio-Pérez,Evelyn
Vargas-Hernández,Genaro
Téllez-Jurado,Alejandro
Gracida-Rodríguez,Jorge
spellingShingle Quintanar-Gómez,Samuel
Abreu-Corona,Arturo
Zamudio-Pérez,Evelyn
Vargas-Hernández,Genaro
Téllez-Jurado,Alejandro
Gracida-Rodríguez,Jorge
PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE
author_facet Quintanar-Gómez,Samuel
Abreu-Corona,Arturo
Zamudio-Pérez,Evelyn
Vargas-Hernández,Genaro
Téllez-Jurado,Alejandro
Gracida-Rodríguez,Jorge
author_sort Quintanar-Gómez,Samuel
title PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE
title_short PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE
title_full PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE
title_fullStr PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE
title_full_unstemmed PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE
title_sort production of medium chain length polyhydroxyalkanoates from cupriavidus necator with beeswax hydrolyzates as carbon source
description ABSTRACT This study demonstrates that using beeswax hydrolyzates (Hw), short- or medium-chain-length polyhydroxyalkanoates (scl-mcl-PHA) can be obtained by a three stages fed-batch fermentation system. The fed batch fermentations were conducted with a strain of Cupriavidus necator. Glucose and ammonium sulphate as carbon and nitrogen sources, respectively, were used in a first and second culture stages. In the third stage (production of scl-mcl-PHA) a fresh medium was fed with Hw as a carbon source at a concentration of 5 g/L, obtaining 8.91 g/L of intracellular scl-mcl-PHA and 13.64 g/L of biomass, which resulted in 65.3 % w/w of scl-mcl-PHA. Using nuclear magnetic resonance, the presence of scl and mcl monomers, 3-hydroxybutyrate (3HB) and 3-hydroxydecanoate (3HD) was determined. The percentage of comonomer incorporation different to 3HB was 4.6 mol %. A glass transition temperature (Tg) of -50 ºC and a melting temperature (Tm) of 79.8 ºC were determined for the scl-mcl-PHA (3HB-co-3HDD).
publisher Universidad Nacional Autónoma de México, Centro de Ciencias de la Atmósfera
publishDate 2018
url http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0188-49992018000300467
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