EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMOR

The effect of Crotalus durissus terrificus (LAURENTI, 1768) venom on the evolution of Ehrlich ascites tumor cells was evaluated. Thus, 30-day-old male mice of the Swiss strain were inoculated intraperitoneally with 1x10<img SRC="http:/img/fbpe/jvat/v3n2/image1947.gif"> tumor cells. Then, 7 groups of animals were formed: 3 control groups (physiological, venom and tumor) and 4 experimental groups that received different doses of venom. The experimental groups received 5 intraperitoneal venom injections on the 1<img SRC="http:/img/fbpe/jvat/v3n2/image1948.gif"> , 4<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 7<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 10<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> and 13<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> days after tumor implantation. On the 14<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif" width="28" height="28"> day, 5 animals from each one of the groups were sacrificed, and the variables such as the total and differential counts of cells in the peritoneal cavity and functional state of peritoneal macrophages by macrophage spreading were evaluated. The other 5 remaining animals were kept in the laboratory for 60 days for observation of their survival percentage. The results obtained were statistically analyzed by the Kruskal-Wallis test at 5% significance level. It was observed that Crotalus durissus terrificus venom increases survival time of mice, but does not increase mortality percentage. This venom also increases the percentage of macrophage spreading. We suggest that snake venoms can cause inhibition of tumor growth by activating the inflammatory reaction, mainly the macrophages, stimulating the production of TNF-<img SRC="http:/img/fbpe/jvat/v3n2/image1950.gif"> , IL-1, IL-6 and IL-8. These cytokines may act on tumor cells by different mechanisms, inducing its complete elimination.

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Main Authors: SILVA,R. J. DA, FECCHIO,D., BARRAVIERA,B.
Format: Digital revista
Language:English
Published: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP 1997
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200008
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spelling oai:scielo:S0104-793019970002000081999-01-11EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMORSILVA,R. J. DAFECCHIO,D.BARRAVIERA,B. venom inflammation tumor growth Crotalus durissus terrificus The effect of Crotalus durissus terrificus (LAURENTI, 1768) venom on the evolution of Ehrlich ascites tumor cells was evaluated. Thus, 30-day-old male mice of the Swiss strain were inoculated intraperitoneally with 1x10<img SRC="http:/img/fbpe/jvat/v3n2/image1947.gif"> tumor cells. Then, 7 groups of animals were formed: 3 control groups (physiological, venom and tumor) and 4 experimental groups that received different doses of venom. The experimental groups received 5 intraperitoneal venom injections on the 1<img SRC="http:/img/fbpe/jvat/v3n2/image1948.gif"> , 4<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 7<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 10<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> and 13<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> days after tumor implantation. On the 14<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif" width="28" height="28"> day, 5 animals from each one of the groups were sacrificed, and the variables such as the total and differential counts of cells in the peritoneal cavity and functional state of peritoneal macrophages by macrophage spreading were evaluated. The other 5 remaining animals were kept in the laboratory for 60 days for observation of their survival percentage. The results obtained were statistically analyzed by the Kruskal-Wallis test at 5% significance level. It was observed that Crotalus durissus terrificus venom increases survival time of mice, but does not increase mortality percentage. This venom also increases the percentage of macrophage spreading. We suggest that snake venoms can cause inhibition of tumor growth by activating the inflammatory reaction, mainly the macrophages, stimulating the production of TNF-<img SRC="http:/img/fbpe/jvat/v3n2/image1950.gif"> , IL-1, IL-6 and IL-8. These cytokines may act on tumor cells by different mechanisms, inducing its complete elimination.info:eu-repo/semantics/openAccessCentro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESPJournal of Venomous Animals and Toxins v.3 n.2 19971997-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200008en10.1590/S0104-79301997000200008
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author SILVA,R. J. DA
FECCHIO,D.
BARRAVIERA,B.
spellingShingle SILVA,R. J. DA
FECCHIO,D.
BARRAVIERA,B.
EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMOR
author_facet SILVA,R. J. DA
FECCHIO,D.
BARRAVIERA,B.
author_sort SILVA,R. J. DA
title EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMOR
title_short EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMOR
title_full EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMOR
title_fullStr EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMOR
title_full_unstemmed EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMOR
title_sort effect of crotalus durissus terrificus (laurenti, 1768) venom on the evolution of ehrlich ascites tumor
description The effect of Crotalus durissus terrificus (LAURENTI, 1768) venom on the evolution of Ehrlich ascites tumor cells was evaluated. Thus, 30-day-old male mice of the Swiss strain were inoculated intraperitoneally with 1x10<img SRC="http:/img/fbpe/jvat/v3n2/image1947.gif"> tumor cells. Then, 7 groups of animals were formed: 3 control groups (physiological, venom and tumor) and 4 experimental groups that received different doses of venom. The experimental groups received 5 intraperitoneal venom injections on the 1<img SRC="http:/img/fbpe/jvat/v3n2/image1948.gif"> , 4<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 7<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> , 10<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> and 13<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"> days after tumor implantation. On the 14<img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif" width="28" height="28"> day, 5 animals from each one of the groups were sacrificed, and the variables such as the total and differential counts of cells in the peritoneal cavity and functional state of peritoneal macrophages by macrophage spreading were evaluated. The other 5 remaining animals were kept in the laboratory for 60 days for observation of their survival percentage. The results obtained were statistically analyzed by the Kruskal-Wallis test at 5% significance level. It was observed that Crotalus durissus terrificus venom increases survival time of mice, but does not increase mortality percentage. This venom also increases the percentage of macrophage spreading. We suggest that snake venoms can cause inhibition of tumor growth by activating the inflammatory reaction, mainly the macrophages, stimulating the production of TNF-<img SRC="http:/img/fbpe/jvat/v3n2/image1950.gif"> , IL-1, IL-6 and IL-8. These cytokines may act on tumor cells by different mechanisms, inducing its complete elimination.
publisher Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
publishDate 1997
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200008
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