Use of weathered diesel oil as a low-cost raw material for biosurfactant production

Use of weathered diesel oil as a low-cost raw material for biosurfactant production

This work aimed to investigate the capability of biosurfactant production by Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, using weathered diesel oil from a long-standing spillage as raw material. The effect of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) on biosurfactant production was evaluated. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h at 27±2ºC. Biosurfactant production was monitored according to the De Nöuy ring method using a Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, experiments were also done applying ultrasound as a way to rupture the cells. For the conditions studied, the cultures did not indicate production of biosurfactants. Results obtained with a hydrocarbon biodegradability test based on the redox indicator 2,6-dichlorophenol indophenol showed that only the commercial diesel was biodegraded by the cultures.

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Bibliographic Details
Main Authors: Mariano,A. P., Bonotto,D. M., Angelis,D. F., Pirôllo,M. P. S., Contiero,J.
Format: Digital revista
Language:English
Published: Brazilian Society of Chemical Engineering 2008
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322008000200006
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spelling oai:scielo:S0104-663220080002000062008-07-03Use of weathered diesel oil as a low-cost raw material for biosurfactant productionMariano,A. P.Bonotto,D. M.Angelis,D. F.Pirôllo,M. P. S.Contiero,J. Biosurfactant Diesel oil Raw material Weathered This work aimed to investigate the capability of biosurfactant production by Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, using weathered diesel oil from a long-standing spillage as raw material. The effect of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) on biosurfactant production was evaluated. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h at 27±2ºC. Biosurfactant production was monitored according to the De Nöuy ring method using a Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, experiments were also done applying ultrasound as a way to rupture the cells. For the conditions studied, the cultures did not indicate production of biosurfactants. Results obtained with a hydrocarbon biodegradability test based on the redox indicator 2,6-dichlorophenol indophenol showed that only the commercial diesel was biodegraded by the cultures.info:eu-repo/semantics/openAccessBrazilian Society of Chemical EngineeringBrazilian Journal of Chemical Engineering v.25 n.2 20082008-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322008000200006en10.1590/S0104-66322008000200006
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Mariano,A. P.
Bonotto,D. M.
Angelis,D. F.
Pirôllo,M. P. S.
Contiero,J.
spellingShingle Mariano,A. P.
Bonotto,D. M.
Angelis,D. F.
Pirôllo,M. P. S.
Contiero,J.
Use of weathered diesel oil as a low-cost raw material for biosurfactant production
author_facet Mariano,A. P.
Bonotto,D. M.
Angelis,D. F.
Pirôllo,M. P. S.
Contiero,J.
author_sort Mariano,A. P.
title Use of weathered diesel oil as a low-cost raw material for biosurfactant production
title_short Use of weathered diesel oil as a low-cost raw material for biosurfactant production
title_full Use of weathered diesel oil as a low-cost raw material for biosurfactant production
title_fullStr Use of weathered diesel oil as a low-cost raw material for biosurfactant production
title_full_unstemmed Use of weathered diesel oil as a low-cost raw material for biosurfactant production
title_sort use of weathered diesel oil as a low-cost raw material for biosurfactant production
description This work aimed to investigate the capability of biosurfactant production by Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, using weathered diesel oil from a long-standing spillage as raw material. The effect of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) on biosurfactant production was evaluated. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h at 27±2ºC. Biosurfactant production was monitored according to the De Nöuy ring method using a Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, experiments were also done applying ultrasound as a way to rupture the cells. For the conditions studied, the cultures did not indicate production of biosurfactants. Results obtained with a hydrocarbon biodegradability test based on the redox indicator 2,6-dichlorophenol indophenol showed that only the commercial diesel was biodegraded by the cultures.
publisher Brazilian Society of Chemical Engineering
publishDate 2008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322008000200006
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