Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma

SUMMARY OBJECTIVE: This study aims to investigate the association between the immunohistochemical expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 and plasma zinc in women with fibroadenoma. METHODS: This cross-sectional study included 37 premenopausal women with fibroadenoma. Waist circumference and body mass index of the participants were measured. Plasma zinc concentrations were determined using atomic flame absorption spectrophotometry. Fragments of breast tissue were fixed and incubated with primary mouse monoclonal antibodies (monoclonal antibodies matrix metalloproteinase -2 -507 and monoclonal antibodies matrix metalloproteinase -9-439). Semi-quantitative analysis of matrix metalloproteinase-2 and matrix metalloproteinase-9 immunoreactivity was performed. Spearman’s test and Friedman’s test were used for statistical analyses. The p<0.05 were considered statistically significant. RESULTS: The average age of the participants was 32.81±9.51 years. The body mass index and waist circumference values were within the normal range. The mean plasma zinc concentration was 42.73±13.84 µg/dL, with 94.6% inadequacy. A statistically significant difference was found between the positive expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 (p=0.0184). There was no significant correlation between the matrix metalloproteinase expression and the plasma zinc levels. CONCLUSIONS: Women with fibroadenoma had hypozincemia and positive expression of metalloproteinases.

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Main Authors: Martins,Luana Mota, Barros,Igor Sabino, Ferreira,Emanuelle de Sousa, Silva Neto,Antonio Gomes da, Dourado,Carla Solange de Melo Escórcio, Oliveira,Victor Alves de, Oliveira,Ana Raquel Soares de, Morais,Jennifer Beatriz Silva, Marreiro,Dilina do Nascimento, Silva,Benedito Borges da
Format: Digital revista
Language:English
Published: Associação Médica Brasileira 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302021000700806
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spelling oai:scielo:S0104-423020210007008062021-10-19Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenomaMartins,Luana MotaBarros,Igor SabinoFerreira,Emanuelle de SousaSilva Neto,Antonio Gomes daDourado,Carla Solange de Melo EscórcioOliveira,Victor Alves deOliveira,Ana Raquel Soares deMorais,Jennifer Beatriz SilvaMarreiro,Dilina do NascimentoSilva,Benedito Borges da Matrix Metalloproteinases Fibroadenoma Zinc SUMMARY OBJECTIVE: This study aims to investigate the association between the immunohistochemical expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 and plasma zinc in women with fibroadenoma. METHODS: This cross-sectional study included 37 premenopausal women with fibroadenoma. Waist circumference and body mass index of the participants were measured. Plasma zinc concentrations were determined using atomic flame absorption spectrophotometry. Fragments of breast tissue were fixed and incubated with primary mouse monoclonal antibodies (monoclonal antibodies matrix metalloproteinase -2 -507 and monoclonal antibodies matrix metalloproteinase -9-439). Semi-quantitative analysis of matrix metalloproteinase-2 and matrix metalloproteinase-9 immunoreactivity was performed. Spearman’s test and Friedman’s test were used for statistical analyses. The p<0.05 were considered statistically significant. RESULTS: The average age of the participants was 32.81±9.51 years. The body mass index and waist circumference values were within the normal range. The mean plasma zinc concentration was 42.73±13.84 µg/dL, with 94.6% inadequacy. A statistically significant difference was found between the positive expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 (p=0.0184). There was no significant correlation between the matrix metalloproteinase expression and the plasma zinc levels. CONCLUSIONS: Women with fibroadenoma had hypozincemia and positive expression of metalloproteinases.info:eu-repo/semantics/openAccessAssociação Médica BrasileiraRevista da Associação Médica Brasileira v.67 n.6 20212021-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302021000700806en10.1590/1806-9282.20201015
institution SCIELO
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Martins,Luana Mota
Barros,Igor Sabino
Ferreira,Emanuelle de Sousa
Silva Neto,Antonio Gomes da
Dourado,Carla Solange de Melo Escórcio
Oliveira,Victor Alves de
Oliveira,Ana Raquel Soares de
Morais,Jennifer Beatriz Silva
Marreiro,Dilina do Nascimento
Silva,Benedito Borges da
spellingShingle Martins,Luana Mota
Barros,Igor Sabino
Ferreira,Emanuelle de Sousa
Silva Neto,Antonio Gomes da
Dourado,Carla Solange de Melo Escórcio
Oliveira,Victor Alves de
Oliveira,Ana Raquel Soares de
Morais,Jennifer Beatriz Silva
Marreiro,Dilina do Nascimento
Silva,Benedito Borges da
Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma
author_facet Martins,Luana Mota
Barros,Igor Sabino
Ferreira,Emanuelle de Sousa
Silva Neto,Antonio Gomes da
Dourado,Carla Solange de Melo Escórcio
Oliveira,Victor Alves de
Oliveira,Ana Raquel Soares de
Morais,Jennifer Beatriz Silva
Marreiro,Dilina do Nascimento
Silva,Benedito Borges da
author_sort Martins,Luana Mota
title Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma
title_short Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma
title_full Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma
title_fullStr Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma
title_full_unstemmed Expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma
title_sort expression of metalloproteinases 2 and 9 and plasma zinc concentrations in women with fibroadenoma
description SUMMARY OBJECTIVE: This study aims to investigate the association between the immunohistochemical expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 and plasma zinc in women with fibroadenoma. METHODS: This cross-sectional study included 37 premenopausal women with fibroadenoma. Waist circumference and body mass index of the participants were measured. Plasma zinc concentrations were determined using atomic flame absorption spectrophotometry. Fragments of breast tissue were fixed and incubated with primary mouse monoclonal antibodies (monoclonal antibodies matrix metalloproteinase -2 -507 and monoclonal antibodies matrix metalloproteinase -9-439). Semi-quantitative analysis of matrix metalloproteinase-2 and matrix metalloproteinase-9 immunoreactivity was performed. Spearman’s test and Friedman’s test were used for statistical analyses. The p<0.05 were considered statistically significant. RESULTS: The average age of the participants was 32.81±9.51 years. The body mass index and waist circumference values were within the normal range. The mean plasma zinc concentration was 42.73±13.84 µg/dL, with 94.6% inadequacy. A statistically significant difference was found between the positive expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 (p=0.0184). There was no significant correlation between the matrix metalloproteinase expression and the plasma zinc levels. CONCLUSIONS: Women with fibroadenoma had hypozincemia and positive expression of metalloproteinases.
publisher Associação Médica Brasileira
publishDate 2021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302021000700806
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