Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia
SUMMARY OBJECTIVE: To assess the impact of the metoclopramide-induced hyperprolactinemia in cellular death and proliferation in the harderian gland of female mice. METHODS: Twenty female mice were divided into two groups of 10 animals each and treated: 0.2 mL of saline solution (controls, Ctr) and 200 µg of metoclopramide (experimental, hyperprolactinemia), both for 50 consecutive days and at 12:00 a.m. On the 50th day, the female were euthanized, and the harderian glands were removed and processed for immunohistochemistry for detected ki67 and TUNEL method. Data were statistically analyzed by unpaired Student’s t test (p<0.05). RESULTS: The harderian gland of the hyperprolactinemia group showed increase in the immunoexpression of Ki67 and TUNEL compared to the Ctr group (p<0.05), and there was no significant difference in the amount of porphyrin in the HPrl group compared to the Ctr group. CONCLUSION: The hyperprolactinemia led to increased cell death in the acini the harderian gland and cell proliferation in the stroma glandular, fact that suggesting a reduction process of cellular activity and fibrosis, which suggests impairment in the functioning of the lacrimal harderian.
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Associação Médica Brasileira
2021
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oai:scielo:S0104-423020210006006502021-09-15Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemiaAraújo,Ariadne Stavare LealVerna,CarinaAraújo Júnior,Osvaldo PereiraSoares Júnior,José MariaSimões,Ricardo SantosBaracat,Edmund ChadaSimões,Manuel de JesusGomes,Regina Célia Teixeira Proliferation, cell Harderian gland Mice Hyperprolactinemia SUMMARY OBJECTIVE: To assess the impact of the metoclopramide-induced hyperprolactinemia in cellular death and proliferation in the harderian gland of female mice. METHODS: Twenty female mice were divided into two groups of 10 animals each and treated: 0.2 mL of saline solution (controls, Ctr) and 200 µg of metoclopramide (experimental, hyperprolactinemia), both for 50 consecutive days and at 12:00 a.m. On the 50th day, the female were euthanized, and the harderian glands were removed and processed for immunohistochemistry for detected ki67 and TUNEL method. Data were statistically analyzed by unpaired Student’s t test (p<0.05). RESULTS: The harderian gland of the hyperprolactinemia group showed increase in the immunoexpression of Ki67 and TUNEL compared to the Ctr group (p<0.05), and there was no significant difference in the amount of porphyrin in the HPrl group compared to the Ctr group. CONCLUSION: The hyperprolactinemia led to increased cell death in the acini the harderian gland and cell proliferation in the stroma glandular, fact that suggesting a reduction process of cellular activity and fibrosis, which suggests impairment in the functioning of the lacrimal harderian.info:eu-repo/semantics/openAccessAssociação Médica BrasileiraRevista da Associação Médica Brasileira v.67 n.5 20212021-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302021000600650en10.1590/1806-9282.20200737 |
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Araújo,Ariadne Stavare Leal Verna,Carina Araújo Júnior,Osvaldo Pereira Soares Júnior,José Maria Simões,Ricardo Santos Baracat,Edmund Chada Simões,Manuel de Jesus Gomes,Regina Célia Teixeira |
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Araújo,Ariadne Stavare Leal Verna,Carina Araújo Júnior,Osvaldo Pereira Soares Júnior,José Maria Simões,Ricardo Santos Baracat,Edmund Chada Simões,Manuel de Jesus Gomes,Regina Célia Teixeira Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia |
author_facet |
Araújo,Ariadne Stavare Leal Verna,Carina Araújo Júnior,Osvaldo Pereira Soares Júnior,José Maria Simões,Ricardo Santos Baracat,Edmund Chada Simões,Manuel de Jesus Gomes,Regina Célia Teixeira |
author_sort |
Araújo,Ariadne Stavare Leal |
title |
Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia |
title_short |
Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia |
title_full |
Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia |
title_fullStr |
Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia |
title_full_unstemmed |
Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia |
title_sort |
imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia |
description |
SUMMARY OBJECTIVE: To assess the impact of the metoclopramide-induced hyperprolactinemia in cellular death and proliferation in the harderian gland of female mice. METHODS: Twenty female mice were divided into two groups of 10 animals each and treated: 0.2 mL of saline solution (controls, Ctr) and 200 µg of metoclopramide (experimental, hyperprolactinemia), both for 50 consecutive days and at 12:00 a.m. On the 50th day, the female were euthanized, and the harderian glands were removed and processed for immunohistochemistry for detected ki67 and TUNEL method. Data were statistically analyzed by unpaired Student’s t test (p<0.05). RESULTS: The harderian gland of the hyperprolactinemia group showed increase in the immunoexpression of Ki67 and TUNEL compared to the Ctr group (p<0.05), and there was no significant difference in the amount of porphyrin in the HPrl group compared to the Ctr group. CONCLUSION: The hyperprolactinemia led to increased cell death in the acini the harderian gland and cell proliferation in the stroma glandular, fact that suggesting a reduction process of cellular activity and fibrosis, which suggests impairment in the functioning of the lacrimal harderian. |
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Associação Médica Brasileira |
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2021 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302021000600650 |
work_keys_str_mv |
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