Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost
Plant wastes present a high cellulose content, which is an ideal organic material for composting. Five strains of thermophiles from processed Brassica waste were isolated, and the hydrolytic activity on various cellulosic biomass substrata and their temperature profiles were determined. 16S rRNA sequencing identified these strains as Thermoactinomyces and Bacillus spp. Maximal cellulase activity corresponded to 2.3 U mL-1 of enzyme. The application of these strains on Brassica rapa residues demonstrates increased total nitrogen content). TA-3, a Thermoactinomycetes sp. strain, performs best among all inoculants, increasing the nitrogen content from 0.74 to 0.91%, and decreasing the carbon content from 15.4 to 12.2%, showing its high efficiency and bioactivity during compositing.
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Escola Superior de Agricultura "Luiz de Queiroz"
2009
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oai:scielo:S0103-901620090003000042009-06-29Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compostChang,Chen-ChinNg,Chang-ChaiWang,Chung-YiShyu,Yuan-Tay cellulose degrading enzymes vegetable wastes Plant wastes present a high cellulose content, which is an ideal organic material for composting. Five strains of thermophiles from processed Brassica waste were isolated, and the hydrolytic activity on various cellulosic biomass substrata and their temperature profiles were determined. 16S rRNA sequencing identified these strains as Thermoactinomyces and Bacillus spp. Maximal cellulase activity corresponded to 2.3 U mL-1 of enzyme. The application of these strains on Brassica rapa residues demonstrates increased total nitrogen content). TA-3, a Thermoactinomycetes sp. strain, performs best among all inoculants, increasing the nitrogen content from 0.74 to 0.91%, and decreasing the carbon content from 15.4 to 12.2%, showing its high efficiency and bioactivity during compositing.info:eu-repo/semantics/openAccessEscola Superior de Agricultura "Luiz de Queiroz"Scientia Agricola v.66 n.3 20092009-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300004en10.1590/S0103-90162009000300004 |
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Chang,Chen-Chin Ng,Chang-Chai Wang,Chung-Yi Shyu,Yuan-Tay |
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Chang,Chen-Chin Ng,Chang-Chai Wang,Chung-Yi Shyu,Yuan-Tay Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost |
| author_facet |
Chang,Chen-Chin Ng,Chang-Chai Wang,Chung-Yi Shyu,Yuan-Tay |
| author_sort |
Chang,Chen-Chin |
| title |
Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost |
| title_short |
Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost |
| title_full |
Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost |
| title_fullStr |
Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost |
| title_full_unstemmed |
Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost |
| title_sort |
activity of cellulase from thermoactinomycetes and bacillus spp. isolated from brassica waste compost |
| description |
Plant wastes present a high cellulose content, which is an ideal organic material for composting. Five strains of thermophiles from processed Brassica waste were isolated, and the hydrolytic activity on various cellulosic biomass substrata and their temperature profiles were determined. 16S rRNA sequencing identified these strains as Thermoactinomyces and Bacillus spp. Maximal cellulase activity corresponded to 2.3 U mL-1 of enzyme. The application of these strains on Brassica rapa residues demonstrates increased total nitrogen content). TA-3, a Thermoactinomycetes sp. strain, performs best among all inoculants, increasing the nitrogen content from 0.74 to 0.91%, and decreasing the carbon content from 15.4 to 12.2%, showing its high efficiency and bioactivity during compositing. |
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Escola Superior de Agricultura "Luiz de Queiroz" |
| publishDate |
2009 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300004 |
| work_keys_str_mv |
AT changchenchin activityofcellulasefromthermoactinomycetesandbacillussppisolatedfrombrassicawastecompost AT ngchangchai activityofcellulasefromthermoactinomycetesandbacillussppisolatedfrombrassicawastecompost AT wangchungyi activityofcellulasefromthermoactinomycetesandbacillussppisolatedfrombrassicawastecompost AT shyuyuantay activityofcellulasefromthermoactinomycetesandbacillussppisolatedfrombrassicawastecompost |
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