Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources

Cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. were used to test plant ability to utilize an alternative to sucrose as carbon source and energy for growth. Glycerol, sorbitol and galactose were the alternative carbon sources tested. Cell suspension cultures established on liquid medium containing sucrose were transferred to culture medium supplemented with sucrose or glycerol, or sorbitol, or galactose as the sole carbon source. Fresh and dry weight increasing and protein content showed marked differences among the different carbon sources used. Sucrose was the best carbon source for all the three plant species tested. Galactose and glycerol promoted slow or no growth of the three studied species. Sucrose in liquid medium promoted initiation of meristemoid formation. Sorbitol, which was ineffective on promoting significant growth, was the only alternative carbon source tested that also promoted this effect.

Bibliographic Details

Main Authors:	Mello,Marcia Ometto, Dias,Carlos Tadeu Santos, Amaral,Antônio Francisco Campos, Melo,Murilo
Format:	Digital revista
Language:	English
Published:	Escola Superior de Agricultura "Luiz de Queiroz" 2001
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000300007
Tags:	Add Tag No Tags, Be the first to tag this record!